败酱草抗呼吸道合胞病毒活性成分及其抗病毒机理的研究

Respiratory syncytial virus (RSV) has long been considered an important cause of severe lower respiratory tract infection in infants and young children throughout the world. Unfortunately, no effective treatment of RSV exists. Therefore,New agents are needed to reduce the impact of RSV. We have studied the anti-viral effect of traditional Chinese midicinal herbs for over ten years and find herba patrinea (a Chinese medicinal herb) has the anti-RSV effect in vitro. In this study, the herba patinea was extracted with hot water, condensed and sterilized. The cytotoxicity of the aqueous extract was tested by adding the diluted extract directly to HeLa cells and its effect on anti-RSV was estimated by the CPEI assay. As a result, the median cytotoxic concentration (CC50 ) of herba patinea was 32mg/ml by morphological observation, the median effective concentration (50% effective concentration, EC50 ) of the herba patrinea against replication of the Long strain of RSV in Hela cells were 1.25mg/ml. The selectivity index ( SI = CC50 / EC50 ) is 25.6. Moreover, herba.patrinea gave a dose-dependent response in inhibiting RSV. In time of addition.experiment, herba patrinea inhibited replication of RSV in HeLa cells when it was added at 0h, 2h, 4h after virus infection. In summary, the results of this study suggest herba patrinea may be a novel anti-RSV drug and it is worthy of further studying.

用化学分离法分离败酱草抗呼吸道合胞病毒活性成分，以细胞培养和动物实验法验证其抗呼吸道合胞病毒作用，在此基础上进行该活性成分抗呼吸道合胞病毒机理研究，为研制新抗呼吸道合胞病毒药物（1或2类中药）和研究抗病毒先导化合物提供科学依据，并可为研究中草药抗病毒机理提供新思路，填补中草药抗病毒机理研究的空白。