抑制独角金内酯生物合成创制矮生、多分蘖高羊茅种质的研究

Strigolactones (SLs) is a novel class of plant hormones that regulates plant height and shoot branching, it has great value for improvement the traits of high-tillering and dwarf phenotype in turfgrass . However, few results about synthesis pathways and biological functions of SLs in turfgrass was reported. In this study, we take tall fescue as research materials, which has been widely used in China. Previously, We found that the phenotype of dwarf and increased tilling was generated by inhibiting SLs synthesis in tall fescue. On this basis, we will identify a crucial gene , FaCCD8, which gets conserved sequences and biological functions in SLs synthesis pathways ,and silence its expression by RNAi, thus generates new germplasm with dwarf and increased tillering phenotype. In this research, we will confirm the synthesis pathways and biological functions of SLs in tall fescue, provide germplasms for new variety breeding, and guide the breeding of other turfgrasses .

独角金内酯是新发现的一类植物激素，在调控植物株高和分蘖等方面发挥着重要作用，对草坪草矮生、分蘖性状改良有非常高的研究价值，但目前还缺乏有关草坪草独角金内酯的研究。高羊茅是国内外应用最为广泛的草坪草种之一，前期研究发现，独角金内酯合成受阻将导致高羊茅植株矮化、多分蘖。在此基础上，项目拟克隆独角金内酯合成途径上一个结构和功能都极为保守的关键基因FaCCD8，结合RNA干扰技术，定向沉默FaCCD8基因的表达，从而创制矮生、多分蘖的高羊茅种质。项目将明确独角金内酯在高羊茅体内的合成途径及发挥的生物学功能，为选育矮生、多分蘖高羊茅品种提供育种材料，同时对其它草坪草矮生、分蘖性状改良也有重要指导意义。