滩羊宰后肌细胞代谢动态演化及其通路转换机制研究

After slaughter and bleeding, animals died ,but the function of organs and tissues did not give over at the same time, various life activities were still being carried out in Cells. Owing to the interruption of the body's respiration and blood circulation, various aerobic biochemical reactions in muscle cells were called off, which will resulting in metabolism differences between postmortem and living body. The research on the metabolism differences of postmortem muscle cell has a positive guiding role not only in the understanding of the mechanism of meat maturation after postmortem but also on the improving of the processing, storage and transportation technology of cold fresh meat. At present, the studies of post-slaughter metabolism are mostly focused on gene regulation of one or several metabolites, both at home and abroad. While , there is no report on the composition and interaction of all genes, mRNAs, proteins, and metabolites ,which could be detected by current group technologies ,by using the postmortem muscle cells as a special life system. Tan sheep is a dominant breed of Ningxia. In this project, cool muscle cells from Tan sheep were used as the research object. Metabolomics was used to study the postmortem metabolite changes and its pathway conversion. Proteomics and metabolomics were linked to study the linkage relations between metabolic changes and the changes of metabolic enzyme. Proteomics and metabolomics were associated with transcriptomics to identify the differential genes involved in biochemical metabolic pathways, as well as the composition, interrelationships, and transformation of genes, mRNA, proteins, and metabolites. Which will revealing the dynamic evolution of muscle cell metabolism and the mechanism of pathway conversion of tan sheep after slaughter , as well as providing theoretical guidance for the quality control of Tan sheep mutton.

动物屠宰放血后机体死亡，而器官、组织的机能并没有同时停止，细胞仍在进行各种生命活动。由于机体呼吸与血液循环中断，肌细胞内的各种需氧性生化反应停止，代谢与活体产生差异。研究宰后肌细胞代谢差异，对了解动物宰后肉成熟机理，改进冷鲜肉加工贮运技术，有着积极地指导作用。目前国内外宰后代谢研究多集中于某个或某几个代谢物的基因调控。以宰后肌细胞为特殊生命系统，研究当前组学技术可检出的全部基因、mRNA、蛋白质、代谢物的构成及相互作用，却未见报道。滩羊是宁夏优势特色畜种。本项目以滩羊冷却肉肌细胞为研究对象，以代谢组学研究宰后代谢物变化及其通路转换，以蛋白质组学关联代谢组学研究代谢变化与代谢酶变化的联动关系，以转录组学关联蛋白质组学、代谢组学确定参与生化代谢途径的差异基因，研究基因、mRNA、蛋白质、代谢物的构成、相互关系及转化。揭示滩羊宰后肌细胞代谢动态演化及其通路转换机制，为滩羊肉品质控制提供理论指导。

滩羊为宁夏优势特色畜种，肉质细嫩，风味独特，深受消费者喜爱。羊肉在贮藏加工过程中会发生一系列复杂的生理、生化、物理变化。本项目采用多组学技术研究滩羊肉贮藏期间品质变化的生化机制及调控因素，通过多组学数据关联分析确定肉品质变化的分子变化、反应历程、调控因素及条件，构建了核心基因-差异代谢物互作网络，鉴定了调控能量代谢转换的核心基因，确定了COX为宰后成熟期间滩羊肉能量代谢转换的关键调控因子，确定了HIF-1在宰后对组织缺氧状态的调控作用。了解滩羊宰后肉成熟机理、改善肉品质，改善冷鲜肉加工贮运技术提供理论指导。