基于构型可控的高密集糖码分子与凝集素的识别作用研究

The interactions of carbohydrate and proteins (lectins) are important recognition events that regulate a myriad of biological and pathological processed. It is significant to investigate the carbohydrate-protein interaction based on the behavior of between characteristic carbohydrate code and specific lectins recognition. .With the aim of gaining insight into the fundamental mechanisms of the highly site-specific carbohydrate-lectin recognition, herein, a series of controllable biocompatible and high density sugar-grafted oligomeric(ethylene glycol) (OEG) multivalent synthetic sugar code complex which have accurately regulated the factors involving the glycosyl categories, the special glycosidation conformation, adjustable linkage, sugar arrangement and fixed ratio of different sugar code, have been be designed in this proposal..In order to validate and develop systematic carbohydrate-protein interaction research based on our designed complexs, the glyco-chip system which grafted this high density sugar code polymer will be employed on the basis of performance-proof chip system in this project. Meanwhile, in view of achiving an application of the designed molecule, and based on the common phenomenon that lectins or different sugar moieties are usually overexpressed on the outer surface membrane of mutated cancerous cells, this project would be directed to practical application based on the performance-proof of this sugar code molecular model..This project leads to comprehensive and systematic understanding the carbohydrate-protein inter-recognition at the molecular level from various carbohydrate-protein recognition behavior questions that help to illustrate the many setting for synthetic polyvalent systems, including molecular configuration, response mechanism, interaction regulation, condition response and signal transformation. This research scheme, including general implementation from molecular design to performance proof and further practical application, provides an elaborate thought and strategy to systematic investigation on carbohydrate-protein interaction.

糖-蛋白(凝集素)识别在多种生物学运行机制中处于核心地位，开展以特征糖码与凝集素的特异性识别行为为基础的糖-凝集素相互作用研究具有重要意义。本项目以糖-凝集素相互作用为研究对象，以多重价键增强效应为手段，通过精确调控分子模型中的糖基种类、糖苷键构型、糖基连接方式、糖基参与聚合比例等因素，设计具有高生物相容性主链下的可控高密集糖码分子模型，并开展基于性能验证的糖-凝集素作用系统研究。同时，课题还将以性能验证为基础将研究引向应用，开展可控高密集糖码分子模型下的药物定向传输应用研究。课题拟从分子构型、应答机制、作用力调节、条件响应、信号转换等各项糖-凝集素识别行为问题方面，在分子水平上全面系统地理解糖-凝集素间互识作用关系。全面实施从分子设计到性能验证再到应用的研究理念，为糖-凝集素相互作用的系统研究提供精细化的思路及方法。