组织蛋白酶调控褐飞虱生长的分子机理

The brown planthopper (BPH), Nilaparvata lugens (Stål), is a serious pest in paddy fields throughout Asia. Different physiologies from BPH populations with different virulence were existed, including development, metamorphosis and reproduction. In this project, firstly, we planted to clone genes that involved in "PIs-cathepsin-JH/MH" pathway. Secondly, the interaction mechanisms of 3 genes including cathepsin and its uptream genes Relish and E93, will be screened with RNAi, yeast two-hybrid system and eukaryotic expression system. Thirdly, molecular and genetic mechanisms of these three genes will be revealed in three populations (TN1, Mudgo and IR56) by whole genome re-sequencing, RNA-seq, qPCR, enzyme activity assay, zymogram analysis and ITRAQ. Finally, the roles of cathespin will be revealed in the development, and genetic mechanism of virulence. The results will provide fundamental resources for the control strategies.

褐飞虱是亚洲水稻上重要害虫，其不同致害性种群生长和发育等生物学特征存在差异。基于已构建的褐飞虱“蛋白酶抑制剂-组织蛋白酶-保幼激素/蜕皮激素”途径，本项目拟克隆这一途径中的重要基因；通过RNAi、酵母双杂交、真核细胞表达系统等技术重点明确组织蛋白酶及其上游的转录因子Relish 、E93等蛋白的互作机制。在探明褐飞虱3个种群（TN1、Mudgo和IR56）生长、变态发育和生殖等生理指标的基础上，重点通过基因组重测序、转录组测序、qPCR、酶活力测定、酶谱、ITRAQ等技术明确组织蛋白酶、Relish 、E93基因变异的分子机理。最后，通过RNAi和致害力测定等技术，拟进一步明确组织蛋白酶在褐飞虱生长和致害性变异中的作用机制。本项目研究结果有望为褐飞虱治理提供基础资料。

褐飞虱是亚洲水稻上重要害虫，其不同致害性种群生长和发育等生物学特征存在差异。基于已构建的褐飞虱“蛋白酶抑制剂-组织蛋白酶-保幼激素/蜕皮激素”途径，本项目克隆这一途径中的组织蛋白酶、bHLH、E93等重要基因；明确了褐飞虱组织蛋白基因与种群、水稻品种的相关性及其基因功能；鉴定出3个调控组织蛋白酶启动子的bHLH基因；鉴定了褐飞虱组织蛋白酶下游调控基因E93在褐飞虱4龄期参与变态发育及生长过程。本项目研究结果有望为褐飞虱治理提供基础资料。. 在本项目的资助下，本课题组发表第一标注论文2篇、第二标准论文3篇；1名研究生获得硕士学位。