重组蝎毒素基因的表达及抗肿瘤活性的研究

A venom gland cDNA library of Chinese scorpion Buthus martensii Karsch was built by ourselves through the technique of molecular biology, and more than 40 new toxin genes were obtained from the library, which. are vital source to develop novel medicines with our own intellectual asset. At present, some of above new genes have been expressed by the designed expression vectors, and structure and function of these corresponding products were studied and indentified. The research results indicated that the recombinant scorpion peptide BmKCT could specifically blocked Cl- channel to repress the reproduction of glioma cells, recombinant BmKAEP and BmKIM were blockers of Na+ channel with the function of anti-epilepsy since they reduced release of stimulant neurotransmitter glutamic acid before the nerve synapse through influencing the opening of Na+ channle, in addition, BmKIM also had the function of anti-arrhythmia and abirritate. All results from this project has laid a strong foundation for theory and application research of anti-cancer, anti-epilepsy, anti-arrhythmia and analgesia with scorpion toxins. Until now, there are 13 SCI papers among 17 published research papers.

运用原核表达系统大量表达三种不同类型分别作用于Na+和K+通道的蝎毒素基因，其产物用谏秆∥逯植煌橹橹丛吹陌┫赴担⒔⒚舾邪┫赴档氖笛槎锬Ｐ停舛ㄖ刈樾舅鼗钚钥怪琢龅幕钚裕窈蠼浞⒄钩尚乱淮蚬こ炭拱┮┪锏於ɑＭ备醚芯拷夜犹烊恢幸┳试粗锌⑿滦碗睦嘤行С煞钟糜诟髦忠赡鸭膊≈瘟频目焖俜⒄埂