部分营养及非营养因子对不同品系大黄鱼蛋白周转调控及分子机理研究

Protein turnover is the solely in vivo biological pathways for the proteins that had been deposited during the growing of animals that finally turn into the edible animal products. The whole progress of protein turnover is regulated by many factors, including nutrition and non-nutrition ones. However, so far, the relevant study on protein turnover in aquatic animals is still rarely reported. In the present study, we will compare the effects of several nutrition and non-nutrition factors on the regulations of protein turnover in large yellow croaker with two different strains, and furtherly to discuss the relevant molecular mechanisms of such regulation. The quality and originations of dietary protein source, fasting treatment and following re-feeding were chosen as representatives of nutrition and non-nutrition factors, respectively. Both the classic methods of fish nutrition and feed research, and modern biological technologies, such as: isotopic tracer method, molecular cloning and fluorescence quantitative PCR will be adopted. As the aim of this research, we hope to obtain the details on the nutrition, physiological, and genetic factors on the regulation of fish protein overturn, and reveal the molecular mechanisms. The success of present research will be definitely helpful on improving the dietary protein utilization, reducing the nitrogen loadings, and establishing the optimal feeding management, and also facilitating the further breeding work targeting for the strains with efficient protein utilization and fast growing performance.

动物体蛋白质周转是体内蛋白质沉积形成动物产品的唯一生物学途径，其过程受到多个营养和非营养因素的调节。截至目前有关水产动物蛋白质周转的研究仍鲜有报道。本研究以2个不同品系的大黄鱼为对象，将传统营养与饲料学研究方法与同位素示踪、分子克隆和荧光定量等多种现代生物技术相结合，研究不同饲料蛋白源品质及来源、饥饿及恢复摄食等对不同品系大黄鱼蛋白质周转代谢的调控，以期从分子水平深入解析以上因素对鱼类蛋白周转代谢的调控及机制。本研究结果将有利于阐明营养因素、生理因素及遗传因素对鱼类蛋白周转代谢的调控及机制，提高大黄鱼对饲料蛋白利用效率及降低氨氮的排放，建立大黄鱼合理的投喂模式，并为选育鱼体蛋白储积速度快、效率高的大黄鱼新品系提供参考。

动物体蛋白质周转是体内蛋白质沉积形成动物产品的唯一生物学途径，其过程受到多个营养和非营养因素的调节。有关水产动物蛋白质周转的研究也逐渐成为热点。本研究以2个不同品系的大黄鱼为对象，结合传统营养与饲料学研究方法、同位素示踪、分子克隆和荧光定量等多种现代生物技术，研究了不同多种饲料蛋白源品质及来源、饥饿及恢复摄食等对不同品系大黄鱼蛋白质周转代谢的调控，从分子水平深入解析了以上因素对鱼类蛋白周转代谢的调控及机制。研究结果可从一定程度上帮助提高大黄鱼对饲料蛋白利用效率及降低氨氮的排放，建立大黄鱼合理的投喂模式，并为选育鱼体蛋白储积速度快、效率高的大黄鱼新品系提供参考。主要成果包括以下几个方面：1）获得了3个调控蛋白质降解途径的关键基因的cDNA序列，预测了其氨基酸序列并结构解析；2）初步探明了营养因素、生理因素及遗传因素对3个调控蛋白质降解途径的关键基因影响的分子机理。