FMR1在小鼠卵巢早期发育中的机制研究

Primary ovarian insufficiency (POI), also known as "premature menopause" or "premature ovarian failure" (POF) refers to ovarian dysfunction that results in infertility to early menopause as the end stage.It poses challenge to women welfare during these years.However,there hasn't been effective method to sustain ovarian function. To elucidate the mechanism of POF can provide theoretical basis for POF therapy which is of great promise in both basic research interests and clinical relevance. We propose to further investigate the functional importance of the interaction between FMR1 and PTEN/Akt signaling pathway in the help with some animal models including FMR1 premutation mice,Akt knock out mice and PTEN condition knock out mice. The mechanism of POF related with FMR1 will be elucidated by biochemical means in vitro and in vivo. This study is expected to enrich current knowledge in POF. More importantly,improved understanding of FMR1 and Akt signaling pathway will provide potential targets for POF therapy that could be applied into current treatment regimens for POF.

原发性卵巢功能不全（POI）也称作卵巢早衰（POF）是已建立规律月经的女性在40岁以前出现持续性闭经的现象，严重危害女性身心健康，目前尚无有效方法维持卵巢功能。认清POF的发病机制，可对POF的治疗提供理论依据。本项目以FMR1为出发点，利用现有的FMR1前突变的转基因小鼠模型，结合Akt或PTEN基因敲除小鼠，运用体内和体外方法，试图阐明FMR1与PTEN/Akt途径的关系，从而深入了解FMR1在小鼠早期卵巢发育中的作用及机制，为临床上卵巢功能早衰疾病的治疗提供有力的理论依据。通过本项目的开展可望加深对FMR1在卵巢早期发育过程中的作用，对POF发病机制有一个全新的解释，并为临床上POF药物的研发和POF的治疗提供可靠的理论依据。

原发性卵巢功能不全（POI）也称作卵巢早衰（POF）是已建立规律月经的女性在40岁以前出现持续性闭经的现象，严重危害女性身心健康，目前尚无有效方法维持卵巢功能。认清POF的发病机制，可对POF的治疗提供理论依据。本项目以Fmr1为出发点，利用现有的Fmr1前突变的转基因小鼠模型，结合Akt或PTEN基因敲除小鼠，运用体内和体外方法，阐明Fmr1与PTEN/Akt途径的关系，从而深入了解Fmr1在小鼠早期卵巢发育中的作用及机制，为临床上卵巢功能早衰疾病的治疗提供有力的理论依据。通过本项目的开展可望加深对fmr1在卵巢早期发育过程中的作用，对POF发病机制有一个全新的解释，并为临床上POF药物的研发和POF的治疗提供可靠的理论依据。