细菌与粘液的相互作用对饮食干预实验性结肠炎的影响研究

Intestinal mucus has two layers. the dense structure of the inner layer can effectively prevent bacteria from invading. Bacteria and enzymes of the body metabolize the mucus surface, and form the relatively loose mucus outer layer. Diet can change the intestinal flora, affect the metabolism of mucus layer. Inflammatory changes occur when the intestinal mucus is not compatible with the metabolic product or incomplete. The change of mucus layer is closely related to the pathogenesis of ulcerative colitis. This project aims to treat normal mice and IEC C1galt1-/- mice with high protein and high fiber diet, observe the susceptibility of experimental colitis, detect the production and degradation of intestinal mucus, bacterias in intestinal mucus, short chain fatty acids and amino acids, study the effect of intestinal bacterias on mucus degradation, and expect to find the effect of the interaction between the bacteria and mucus on the diet to experimental colitis.

肠道粘液分为两层，内层结构致密能有效阻止细菌的通过，细菌和机体的酶不断代谢粘液表面，形成相对疏松的粘液外层。饮食可以改变肠道菌群，影响粘液层的代谢。当肠道粘液层接触不适应的代谢产物或粘液层不完整，将出现炎症改变。粘液层的改变与溃疡性结肠炎的发病密切相关。本项目拟用高蛋白、高纤维饮食干预普通小鼠和IEC C1galt1-/-小鼠，观察其对实验性结肠炎的易患性，并检测肠道粘液生成和降解、肠道粘液菌群、短链脂肪酸和氨基酸，研究肠道菌群对粘液的降解作用，期望发现菌群与粘液的相互作用对饮食干预实验性结肠炎中的影响。