高压电晕电场对微生物诱变效应的研究

To better understand the biological effects of high-voltage corona field, expanding the application and study a new means of mutation breeding. A new experimental device which can produce the high-voltage corona electric field will be developed. The design parameters and the electric field characteristics of the device will be measured and analyzed. The strength of electric field，duration and dosage for the mutagenesis bacteria will be explored by evaluating the lethal and mutation regularity of bacteria. Using the high-voltage corona electric field as mutagenic agent to treatment lactic acid bacteria of silage、γ-Poly-glutamic acid-Bacillus natto and spirulina，based on different selection criteria for different strains，the mutant strain will be obtained. The hereditary stability of mutual strain will be detected after sub-culturing several times. The fermentation technology in the seeding tank was optimized. The different microbe are irradiated by corona discharge, that mutagenic effects will be discussed respectively. It provides the mutagenic parameters and the new mutation means. These results will provide high yield strain for economic development and development of animal husbandry.

为深入了解高压电晕电场生物效应，探讨其作为微生物诱变育种的一种新手段。根据电晕电场的放电特性，设计高压电晕电场处理装置。测量并分析此装置中电场设计参数和电场特性。研究电晕电场强度和处理时间对微生物的致死规律和突变规律，获得最佳电场处理剂量。对青贮剂乳酸菌、γ-聚谷氨酸产生菌-纳豆芽孢杆菌、螺旋藻进行诱变处理，根据不同菌种各自的筛选条件，选育获得突变株。经多次传代，检测其遗传稳定性。对试验获得的优良菌株进行中试，优化诱变菌株生产工艺流程。确定电晕电场对不同类型微生物的诱变效果，为微生物诱变育种工作提供一种新的诱变手段和诱变参数。为经济开发和畜牧业发展提供高产菌株。

为深入了解高压电晕电场生物效应，探讨其作为微生物诱变育种的一种新手段。根据电晕电场的放电特性，设计了高压电晕电场处理装置。研究了电晕电场强度和处理时间微生物的致死规律和突变规律，获得最佳电场处理剂量。对青贮剂乳酸菌、γ-聚谷氨酸产生菌—纳豆芽孢杆菌、螺旋藻、出芽短梗霉进行了诱变处理，根据不同菌种各自的筛选条件，选育获得突变株。经多次传代，检测其遗传稳定性。通过对四种不同微生物进行的诱变研究均表明有较好的诱变效果，说明电晕电场处理诱变作为一种诱变育种的手段是切实可行的。为经济开发和畜牧业发展提供了高产菌株。