基于能量代谢的拔罐疗法量效规律研究及神经-免疫调节启动机制探索

Cupping therapy and acupuncture are both excellent traditional Chinese therapy, but the role of the law is unclear, mechanism of the rescarch is not taken seriously..The overseas and domestic research and our previous work have shown that cupping therapy can cause changes in the local microenvironment of the certain acupoints.We think cupping therapy can promote the level of energy metabolism in point area and active the interaction adjustment of neuro-immune.The project comprehensive adopts traditional Acupuncture&Moxibustion theory,Systematic Biology,neurophysiology,histomorphology,bioinformatics and other techniques and methods,take the cold coagulation and blood stasis rabbits and CFS patients as model,combines the pathological models with physiological state ,and animal experiments with human trials, observes micromorphology changes of acupoint area tissue caused by cupping time and cupping negative pressure, explores the interaction adjustment of neuro-immune of cupping therapy, provides scientific basis for the clinical application of cupping therapy safely and effectively.

拔罐疗法与针灸疗法同属中华民族优秀的传统疗法，但作用规律不清，机理研究未受到重视。国内外现有研究和本课题组前期工作表明，拔罐能够引起穴位局部微环境的变化。我们认为拔罐疗法是通过影响穴区微循环的能量代谢，启动神经-免疫调节而产生拔罐效应的。本项目综合采用中医针灸学、系统生物学、神经生理学、组织形态学、生物信息学等技术与方法，以寒凝血瘀证家兔为病理模型，以慢性疲劳综合症(CFS)人群为研究对象，病理模型与生理状态相结合，动物实验和人体试验相结合，观察拔罐时间、压力对穴位局部能量代谢的影响，探索拔罐疗法神经-免疫调节启动机制，为拔罐疗法临床合理应用及干预亚健康状态提供科学依据。

拔罐疗法是一种创伤性物理刺激，能够拔罐引起穴位局部微循环的能量代谢和微环境的变化，包括调节穴区局部神经递质、免疫因子表达，从而启动穴区神经-免疫调节而产生拔罐效应。在以上认识的基础上，本项目以缺氧大鼠和慢性疲劳综合症患者为研究对象，观察拔罐不同时间和压力对疗效和局部能量代谢的影响；综合采用分子生物学、蛋白质组学、生物信息学、复杂网络分析等技术，研究拔罐对神经、免疫相关物质的影响，探索拔罐疗法神经-免疫调节启动机制和拔罐耐缺氧作用机制，为拔罐疗法的临床合理应用及干预亚健康状态提供科学依据。