复合型生物活性骨水泥的研制及临床应用基础研究

Calcium Phosphate Cements (CPC) was a novel and superior biomaterial for bone repair. The superiority lay in that CPC had some unique properties which common materials such as PMMA didn't have as well as all properties necessary for implanted materials. These unique properties included bioresorbability, excellent biocompatibility, slight exothermic reaction, tight interface, sustained releasing of drug and being able to be molded at will. However, CPC biodegraded and colonized by new bone too slowly, and this intrinsic drawback limited severely its clinical application. Bone Morphogenetics Proteins (BMP) was a cytokine which had definite osteoinductive activity and able to accelerate the regeneration and repair of bone. But an ideal carrier or delivery system was absolutely necessary otherwise BMP could not exert its osteoinductive activity. The ideal carrier for BMP had been sought for decades but no satisfactory one had been found. In view of the excellent properties and intrinsic drawback of CPC and BMP and the coincident complementarity between CPC and BMP, we planed to compound recombinant human BMP-2(rhBMP-2) with CPC to a novel bioactive material. Because loading too much rhBMP-2 in CPC might decrease the biomechanical index inevitably while too little rhBMP-2 might result in low bioactivity, two designs of the CPC/rhBMP-2 compound were drawn. One design was to keep the biomechanical properties mainly and to be used for bone filling, so relatively less rhBMP-2 be loaded in compound. The other was to possess enough bioactivity of rhBMP-2 and the biomechanical index could be neglected to some extent if necessary, so much more rhBMP-2 be loaded and this material mainly be used to treat nonunion of fractures and accelerate bone healing.Five experiments have been had and we found that CBAB compounded in ratio of 1 g CPC powder to 5 mg rhBMP-2 degraded and was colonized by new bone more rapidly than pure CPC. CBAB might be used effectively for bone repair and fracture healing in clinic.

磷酸钙水泥CPC是新型骨修复材料，骨形态发生蛋白BMP有良好的诱导成骨作用。本项目研究BMP对CPC力学性能的影响和CPC对BMP生物活性的影响，BMP促进CPC的骨替代和CPC 对BMP的菏妥饔谩Ｖ荚谘兄瞥杉确⒒覥PC的骨填充和缓释作用，又利于发挥BMP诱导成骨作用的复合蜕锘钚怨撬郈BBC,应用于骨缺、骨不连和骨折的治疗。