基于角蛋白序列差异的角类中药材专属性鉴别方法研究

Traditional Chinese medicine used Cornua are epidermal accessory tissues of some of animals like Cornu Saigae Tataricae, Cornu Bubali, cornu caprae hircus, cornu cervi, etc. With the seriously shortage of the cornu resources, there arise a lot of quality problems relate to counterfeit products. For example, the powders of Cornu Saigae Tataricae, which are very rare and expensive now days, are often illegally adulterated with some cheap ones, so it is very clear that a set of specific and sensitive analytical methods are needed urgently for the market surveillance of these products. It is well known that different animals have different AA sequencies of their keratins. Based on the previous research accomplishments, this project aim to search and find the characteristic peptides from the trypsin digested samples of various cornua by the HPLC-MS/MS. The characteristic peptides were then determinated and analysed of their AA sequencies and subjected to chemical synthesized. Hence, A set of HPLC-MS/MS methods would be established to identification of cornua from various origins.

角类药材属皮肤的衍生组织，是我国特有的一类动物来源的中药。目前，由于资源紧缺，导致市场上角类药材假冒伪劣现象较为严重，质量参差不齐，市场秩序非常混乱。特别是针对羚羊角粉等已失去了完整外观性状的珍稀名贵药材的掺伪、造假现象更为严重。然而，由于长期缺乏专属性鉴别方法和高灵敏度的检测技术，面对以上质量问题，药品检验和监管部门往往束手无策。本课题拟在已有的研究基础上，根据不同动物的角蛋白其氨基酸序列必然存在一定的差异，采用液质联用多肽识别技术和数据处理软件对酶解后的角类样品（主含多肽混合物）进行分析处理，寻找并发现各角类样品的特征肽段，同时借用近年来蛋白质及多肽序列研究的技术方法和成果，从分子层次研究各角类药材的主体组分性质及具有专属性鉴别意义的特征多肽成分，从而建立基于角蛋白序列差异的角类药材真伪鉴别方法。以期从根本上为角类药材的质量控制和评价提供技术依据。

角类药材属皮肤的衍生组织，是我国特有的一类动物来源的中药。目前，由于资源紧缺，导致市场上角类药材假冒伪劣现象较为严重，质量参差不齐，市场秩序非常混乱。特别是针对羚羊角粉等已失去了完整外观性状的珍稀名贵药材的掺伪、造假现象更为严重。然而，由于长期缺乏专属性鉴别方法和高灵敏度的检测技术，面对以上质量问题，药品检验和监管部门往往束手无策。本课题在已有的研究基础上，根据不同动物的角蛋白其氨基酸序列必然存在一定的差异，采用液质联用多肽识别技术和数据处理软件对酶解后的角类样品（主含多肽混合物）进行分析处理，寻找并发现各角类样品的特征肽段，同时借用近年来蛋白质及多肽序列研究的技术方法和成果，从分子层次研究各角类药材的主体组分性质及具有专属性鉴别意义的特征多肽成分，从而建立了基于角蛋白序列差异的角类药材真伪鉴别方法。为角类药材的质量控制和评价提供技术依据。