低氮胁迫对丹参酮类物质积累影响及对其合成酶基因表达的研究

Salvia miltiorrhiza, the dried roots of the plant S.Miltiorrhiza Bunge, is a famous tradition medicine in Chinese, S.miltiorrhiza has the function of promotes blood flow to overcome blood stasis and cool the blood to resolve abscesses, bactericide, anti-inflammatory. Besides, S.miltiorrhiza can be used to treat the cardiovascular disorders in recent years. With the requirement increasing, it is very important to improve the content of S.miltiorrhiza and analyze the corresponding biosynthesis pathway of tanshinones. . Tanshinones in S.miltiorrhiza (Salvia miltiorrhiza Bunge) are liposoluble components, has a very important effect and clinical pharmacology. That tanshinones substances contents does not meet the medicinal requirements directly affect production of S.miltiorrhiza. At present, our preliminary study has showed the content of Tanshinones in lack or low nitrogen nutrition stress conditions is higher, compared to those growing in adequate and high nitrogen nutrition, but the pathway of the nutrient-deficiency stress inducing tanshinone substances synthesis has not yet been revealed, this will restrict the technology of planting S.miltiorrhiza with a high tanshinones level. The study would systematically study the effects of low nitrogen stress on tanshinone substance accumulation, analysis time model and the tissue expression pattern of tanshinone biosynthesis gene. The study would furtherly clear the effects of low nitrogen stress on tanshinone substance accumulation, and investigate the correlation of tanshinone substance accumulation under low nitrogen stress and tanshinone biosynthesis enzyme gene expression. This lay a foundation for the establishment of efficient production of tanshinone substances in cultivation technical system. Meanwhile, the research of this project provide an important reference for revealing the molecular mechanisms of the composition medicinal plant producing secondary metabolites affected by nutritional stress.

丹参酮是丹参（Salvia miltiorrhiza Bunge）的脂溶性成分，具有非常重要的药理和临床作用。丹参酮类物质含量不符合药用要求直接影响着丹参药材生产。我们初步研究发现丹参根中丹参酮类物质含量在低氮胁迫条件下的比较高，但是这种营养胁迫影响丹参酮物质合成途径分子机理至今尚未揭示。本项目将系统地进行低氮胁迫对丹参酮类物质积累影响的研究，并分析丹参酮合酶基因的胁迫应答时间模式及组织表达模式。本研究将进一步明确低氮胁迫对丹参酮物质积累影响，探讨低氮胁迫对丹参酮物质积累与丹参酮合成酶基因表达的相关性。研究将为高效生产丹参酮物质栽培技术体系的建立奠定基础，同时对药用次生代谢产物受营养胁迫影响后的分子调控机制揭示也有重要借鉴意义。

丹参酮是丹参（Salvia miltiorrhiza Bunge）的脂溶性成分，具有非常重要的药理和临床作用。丹参酮类物质含量不符合药用要求直接影响着丹参药材生产。我们初步研究发现丹参根中丹参酮类物质含量在低氮胁迫条件下的比较高，但是这种营养胁迫影响丹参酮物质合成途径分子机理至今尚未揭示。本项目将系统地进行低氮胁迫对丹参酮类物质积累影响的研究，并分析丹参酮合酶基因的胁迫应答时间模式及组织表达模式。本研究将进一步明确低氮胁迫对丹参酮物质积累影响，探讨低氮胁迫对丹参酮物质积累与丹参酮合成酶基因表达的相关性。研究将为高效生产丹参酮物质栽培技术体系的建立奠定基础，同时对药用次生代谢产物受营养胁迫影响后的分子调控机制揭示也有重要借鉴意义。结果表明无氮和低氮条件促进隐丹参酮、丹参酮ⅡA、丹参酮I及总丹参酮含量提高，转录组分析表明低氮胁迫条件丹参酮合成途径关键酶基因表达上调，选取12个基因进行qRT-PCR验证，转录组基因表达数据较为准确，代谢组分析表明低氮胁迫条件丹参根的萜类代谢产物上调。本项目实施发表1篇论文，准备投稿2篇，申请专利1项。