基于ADME和灰色关联分析的地榆制炭止血物质基础和炮制原理研究

In the research “The Study of the Mechanism of Diyutan Processing” ( 30772786) supported by NSFC, we found that the enhancement of haemostatic effect of Carbonized Sanguisorbae were related with the change of tannin composition. On this basis, we propose a working hypothesis, when Sanguisorbae Radix was processed, the content of macromolecules tannin reduced, from which the tannin unit class composition of strong haemostatic activity was produced. This change can also effect on the absorption, metabolism and elimination of effective components, improve bioavailability, and then enhance the haemostatic effect. In this project, we choose tannin of Carbonized Sanguisorbae Radix as the research object. In the way of grey correlation analysis and ADME methods, the dynamic changes of tannin composition and the process of forming or combination of active ingredient to stop bleeding will be studied. We will also study the absorption and metabolic processes in the body. Through these studies, the material basis of haemostatic activity of Carbonized Sanguisorbae Radix will be clarified. These study may be significant to help rich the Charcoal bleeding theory of traditional Chinese medicine and expand the research ideas of processing principle of Chinese medicine theory research is of great significance.

本课题是已完成国家自然科学基金项目“地榆炭炮制原理研究”（30772786）的后续研究，我们发现地榆制炭后止血作用的增强与鞣质类化学成分“质与量”的变化相关。故在此基础上提出工作假说：“地榆鞣质在制炭过程中，大分子的鞣质类成分明显减低，解离和水解产生了具有较强止血活性的鞣质单体类成分和成分群组合，影响地榆鞣质止血有效成分吸收、代谢和消除，提高生物利用度，增强止血作用。”本课题拟以地榆制炭前后鞣质为主要研究对象，动态追踪化学成分变化，与药效试验进行数据灰色关联分析，结合ADME方法，解析制炭对地榆中鞣质类成分动态变化和形成止血有效成分或成分群组合的过程，以及在体内的吸收、代谢过程，解析地榆“制炭增强止血作用”的物质基础，阐释炮制原理。本研究采用ADME、灰色关联分析等方法，深层次的从体内外阐释地榆炭炮制原理，对丰富中药制炭止血理论，拓展中药炮制原理研究思路具有重要意义。