Bc1-2基因家族与肾虚关系的研究

The bcl-2 knockout mice demonstrated fulminant lymphoid apoptosis, polycystic kidneys, hypopigmented hair, as well as abnormalities in development, bone formation and life span which were typical syndrome as deficiency of Kidneys in traditional Chinese medicine. Thus, the Chinese concept of Kidney, which is different from that of western medicine, might be related to the in vivo roles of bcl-2 family. And the traditional Chinese herbal formulates dedicating for affecting the functions of Chinese Kidney might have effects on this gene family. To test this hypothesis, we boservated the changes of expressing levels of Bcl-2 /Bax during aging, a processed related to decline of the functions of Kidenys in TCM, and the Bcl-2/Bax proteins were also compared between patients complaint of deficiency in Kidenys and those who were not. To further test our thinkings, the effects of Jing Gui Shen Qi Wan (Jin Gui Pills for Tonifying Kidney-energy ), a classic herbal formulates believed to affect functions of Chinese Kidneys on expression of Bcl-2 family were also observed. The resultes were that the expression of Bcl-2 in peripheral lymhhoctes were decreased with age, while the expressions of Bax or Fas increased, and the Bcl-2 protein level were lower in patients complaint of deficiency of Kidneys functions than those without such syndrom, the reliefing syndrom treatment could decreased expressions of Bax. The blood were collected from the volunteers before and after their taking the Jin Gui Shen Qi Wan (Jin Gui Pills for Tonifying Kidney-energy ), after then, the plasma were collected and added into in vitro culture systems of lymphocytes taken from another persons, the apoptotic rates of which were quantified after 9 hours by flow cytometric analysis, based on annexin V labeling in conjunction with PI staining. The results indicated that, after taking the pills, the fluorescing intensities of Bcl-2 were increased while those of Bax and decreased; the fluorescing intensities of Bcl-xL had no obvious changs. The apoptotic rates in cultured lymphocytes were decreased in presence of the serum taken from volunteers after drug use (n=9, P<0.05). Our results upported our idea that the concept of Chinese kidneys minht be related to the in vivo roles of bcl-2 family and the Jin Gui Shen Qi Wan could affected the expressions of Bcl-2 members and the serum after taking this drug had effects on natural apoptosis, which provided a useful way to identify some valuable compounds that could affects the bcl-2 family and apoptosis. Additionally, our results also shin a new light on understanding the unusual organ concept of ancient Chinese, which is key for integrating this alternative medicine into the mainstream medicine.

Bcl-2基因敲除小鼠出现典型肾虚表现：形体小、骨发育不良、外耳小、毛发浅、生殖能力陆怠⒍嗄疑觥⒚庖吡ο陆怠⒃缲病Ｆ渌柿辖徊较允荆珺cl-2家庭成分表达异常，及补肾药物作用是否与调节该家庭表达有关，研究Bcl-2家庭与中医肾本质关系。