ZD制剂对竞技运动赛马肢体非开放性软组织损伤（动物模型）作用机制的研究

The modern athletics horses is rising rapidly with the development of the our country's economy. The non-open soft tissue injury in hoof and limb of athletics horses is the most common disease when racing horses are in the movement. According to statistics that it's morbidity is 70 persent in all surgical diseases. Nonsteroidal anti-inflammatory drugs therapy is the main method of treating soft tissue injury in athtetics on abroad .The clinical study shows that the long-term administration of NSALDs can induce many kinds of adverse reactions. It's also hard to eradicate the pathogenesis so that the result shows that horses was forced to eliminated. The traditional Chinese veterinary medicine accumulates many experiences about the treating acute soft tissue injury. One of the characteristics is the external application. However its mechanism is less known and reported in the aspects of foreign atheltes horses. This subject utilizes medicinal extract for exterior application of the ZD preparation to study the medical pharmacodynamics in non-open soft tissue injury of animal model. the effect of the square of medicine is confirmed from the aspects of organization morphology hemodynamic blood vessels permeability inflammation cell apoptosis anti-inflammatory molecules. The mechanism was discussed and apply to athletes horse clinical, To provide theoretical and practical basis that chinese herba preparation treat no-open injury hoof and limb of athletics horses is developed as soon as possible.

随着经济的发展，我国竞技运动赛马正迅速兴起，而赛马在运动中常发疾病是肢蹄的软组织损伤。据统计，该病占外科疾病的70%以上。国外目前对赛马肢蹄软组织损伤治疗主要采用非甾体类药物（NSALDS），临床研究表明：长期应用该类药物会产生褚多不良反应且难以去除病因，使赛马被迫淘汰。祖国中蒙医学在急性软组织损伤治疗中积累了丰富的经验，中蒙药是治疗该病的特色之一，但目前对赛马的作用机制研究甚少，国内外赛马未见有应报道。 本课题是筛选多种中蒙药制成ZD制剂，应用该制剂对赛马肢蹄非开放性软组织损伤的动物模型进行药效学研究。从组织形态学、血液动力学、血管通透性、炎症介质、细胞调亡、抗炎分子等方面验证其疗效，探讨其机制并应用于赛马临床。为尽快研制出治疗赛马肢蹄非开放性软组织损伤的中蒙药制剂提供理论和实践依据。

摘 要.我国的马产业正处在由传统役用向现代马业快速转折时期，竞技运动赛马正迅速兴起，成为人们最受欢迎的文化体育运动之一。而马匹在竞技运动中常发生肢蹄非开放性软组织损伤。目前国内外对赛马肢蹄非开放性软组织损伤治疗均采用非甾体类抗炎药（NSAIDs），长期使用该类药物易造成马匹胃肠道损害、形成血栓等诸多不良反应且难以去除病因。中医中药在对非开放性软组织损伤的治疗上独特之处。非开放性软组织损伤本质是“伤筋”、“血瘀”，血瘀是产生炎症、肿胀和疼痛的主因，通过活血畅通血脉，去瘀生新，血流运行通畅可达消肿止痛抗炎之功效。因此镇痛、抗炎是中药活血化瘀、消肿止痛的重要体现。ZD制剂是外用于非开放性软组织损伤的中药复方，具有起效快、毒副作用小、无兴奋剂等特点。因此，研制开发一种透皮性好、疗效高、副作用小、便于携带的ZD制剂医治运动竞技赛马非开放性软组织损伤具有非常重要的意义。前期李云章已将该制剂的水煎剂应用本地马属动物肢蹄非开放性软组织损伤的临床治疗，收到较好疗效，但对其作用机制未进行过深入研究。本项目是通过现代科技手段将实验动物小白鼠、蒙古马造成非开放性软组织损伤模型，将ZD制剂应用于模型的不同实验中，通过组织病理学、镇痛、抗炎、活血化瘀、毛细血管通透性、血液流变学、炎症介质、细胞凋亡等观察；通过对炎性分子NO与TNF-α、COX-2、cAMP、NO、IL-1β、IL-6、TNF-αSAA、LA、5-HT、bFGF、VEGF等检测；通过马模型临床评估、电镜观察、血液生化、超声、红外热成像等跟踪检测及赛马病例治疗，多方面数据表明：ZD制剂对非开放性软组织损伤有明显的消肿、止痛、抗炎和促进组织修复作用，为探明该药膏的作用机制提供了科学完善理论和实践依据，它对尽快开发国产中药治疗赛马肢蹄非开放性软组织损伤具有重要的意义。