散寒活血祛白方及其拆方对人黑色素细胞TLRs及其信号转导通路影响的研究

Based on the theory of conghanlunzhi, we have shown that Sanhan huoxuequbai recipe have significant effect on treating vitiligo. In order to further probe the action sites of this recipe on treating vitiligo, and to explore the pathogenesis of vitiligo, we propose that TLRs may play an important role in the pathophysiology of vitiligo. Targeting on TLRs, we hypothesize that it will be a novel treatment strategy to increase or decrease the expression of TLRs, or to modulate its downstream signaling transduction pathway. Based on previous work on TLRs and downstream signaling transduction pathway supported by NSFC, we are planning to construct vitiligo cell line, explore the effect of traditional chinese medicine on the expression of TLRs, by using techniques including cell biology, molecular biology, and immunology. Our study will provide novel mechanisms to explain the pathogenesis of vitiligo and further provide the scientific basis for traditional chinese medicine in the treatment of vitiligo.

项目组基于从寒论治理论，采用散寒活血祛白方治疗白癜风取得较好的临床疗效，为进一步探讨其作用靶点以及白癜风的发病机制，我们假设TLRs在白癜风的发病机制中起着重要的作用，以TLRs为靶位，抑制或激活TLRs的表达或调控其信号转导通路，可能是白癜风新的治疗策略，在前期国家自然科学基金研究TLRs及其信号转导通路的基础上，构建体外人黑色素细胞培养模型，应用细胞生物学、分子生物学、免疫学等多学科的研究方法和技术，研究TLRs及其信号转导通路在黑色素细胞的表达和中药的干预作用，从全新的视角揭示白癜风发病的分子机制，为开发治疗白癜风的中药提供科学依据。

项目组基于从寒论治理论，采用散寒活血祛白方治疗白癜风取得较好的临床疗效，为进一步探讨其作用靶点以及白癜风的发病机制，我们假设 TLRs 在白癜风的发病机制中起着重要的作用，以 TLRs 为靶位，抑制或激活 TLRs 的表达或调控其信号转导通路，可能是白癜风新的治疗策略，在前期国家自然科学基金研究 TLRs 及其信号转导通路的基础上， 构建体外人黑色素细胞培养模型，应用细胞生物学、分子生物学、免疫学等多学科的研究方法和技术，研究 TLRs 及其信号转导通路在黑色素细胞的表达和中药的干预作用，从全新的视角揭示白癜风发病的分子机制，为开发治疗白癜风的中药提供科学依据。