鞭角华扁叶蜂神经内分泌系统对其生态适应能力的研究

Chinolyda flagellicornis (F. Smith) is the major pest of shelter-forest of Yangtse Rive. The sustainable management of this pest must be predicated on studying on the ecological adaptation of neurosecretory system of Chinolyda flagellicornis. In the thesis, the physiology bases of ecological adaptation of prepupa were studied comprehensively and systematically with the methods of Fluorescence Spectrophotometer, micro- and ultrastructure observation, RIA, and HPLP analysis, and the ecological adaptation mechanism of neuroendocrine regulation was also investigated. The results showed that Chinolyda flagellicornisi, passed summer and winter as the stage of diapause prepupa, and overall lasted for more than 300 days. With the development of diapause, the titres of glycogen in the fat body, protein in the haemolymph, and nucleic acid of prepupa dropped gradually, while the titres of haemolymph trehalose were going up. The transforming relationship effected by the temperature and the diapause stages between glycogen and trehalose was found. The amount of titres of glycogen in the fat body, protein in the haemolymph, nucleic acid, the titres of haemolymph trahalose in female prepupa were higher than that in male prepupa. There was a positive correlation between the activities of protective enzyme SOD, CAT and instar, while the relationship between POD activities and instar showed a negative correlation. Compared with the larva, the activities of the three protective enzymes reduced sharply. The activities of protective enzyme in female prepupa were higher than that in male prepupa. With development of larva, the oxygen consumption increased gradually, while the metabolism rate reduced. In 20~30℃ conditions, there was a positive correlation between larva metabolism rate and temperature for 5, 6 instar. While the metabolism rates of prepupa during diapause stage were very low, and had nothing to do with the surrounding temperature. The metabolism rates of male prepupa were higher than that of female prepupa obviously. JH and MH regulated the diapause of Chinolyda flagellicornis. In the last instar, the JH titres of haemolymph dropped sharply, but still maintained in mid-titres. While, for MH titres, there were two separated MH titres peak values. In prepupa, the JH and MH titres of haemolymph maintained in a higher level (about 20ng/mL). From about one week before pupation, the JH titres of haemolymph dropped gradually, while the MH titres going up, and like last instar, formed two separated MH titres peak values. The JH and MH titres in the female prepupa were similar to that of in male prepupa. The results of anatomy of prepupa showed that there were no shrivel indications of glands. Which indicated that the glands maintained activity during the diapuse prepupa stage, and this identical to the results of JH and MH titres analysis. The diapause meachnism of Chinolyda flagellicornis were elucidated for the first time in this study, and the physiology bases of ecological adaptationof prepupa were definituded. This will enrich the theory of diapause mechanism of insect, and supply more important data for comprehending the character of diapauseof insect

鞭角华扁叶蜂是长江流域防护林重要的害虫，严重发生时可造成相当面积树木的死亡。深入研究其成灾生态适应能力的神经内分泌调控机制是实现对该害虫持续治理的基础。本项目拟运用实验生理生化学和生态学方法与技术，系统研究鞭角华扁叶蜂种群动态的环境控制因子及生态适应的生理生化基础与神经内分泌调控机理等问题，提出治理该虫的新策略新手段。.