落叶果树通过细胞分裂素基因工程改良重要农艺性状研究

Through Agrobacterium tumefaciens-mediated transformation system, kiwifruit and apple were transformed with ipt gene cloned from plasmid Ti of Agrobacterium tumefaciens. Plant cytokinin were improved in transgenic plants and some important cropping characters were improved, including tree form, crotch angle and growth vigor, for dwarf culture and greenhouse culture. For the time being, a high efficient, reproducible Agrobacterium tumefaciens mediated gene transfer system were developed in kiwifruit and apple. large kanamycin-resistant plants of the genetically transformed kiwifruit and apple were obtained at very high frequency from the optimized system in the experiments with Agrobacterium tumefaciens, which contained ipt gene.

通过基因工程技术手段将来自农杆菌Ti质粒ipt基因、Ri质粒rolC基因利用农杆菌介导转入几种主要落叶果树，提高植株内源细胞分裂素水平，获得在树体大小、分枝特性、果实品质等农艺性状上有重大改良的完整转基因植株，最终选育得到一批适于矮化密植和设施栽培，容易实现果树生产的早产、丰产、稳产与优质的果树品种和砧木。.