基于水通道蛋白探讨电针改善超促排卵PCOS大鼠卵母细胞质量和发育潜能的作用与机制

Oocyte quality is one of the most important factors influencing the success rate of assisted reproductive technology in polycystic ovary syndrome (PCOS). Ovarian local regulatory factor aquaporin 9 (AQP9) plays an important role in oocyte maturation, fertilization, and subsequent embryo development.Many studies and previous studies have shown that acupuncture can improve the PCOS assisted reproductive rate, but the specific mechanism is still not clear. In this study, PCOS rat model as the object of study, electroacupuncture as a means of intervention,by the application of bioinformatics,molecular biology, reproductive biology and other interdisciplinary means，the project intends to investigate the expression of AQP9 in ovarian tissues of ovulation in ovariectomized PCOS rats and the regulation of AQP9 on oocyte quality,and observes the effect of electroacupuncture on the quality and development potential of oocytes in PCOS rats induced by Superovulation,in order to explore its mechanism and target from AQP9 signal transduction pathway ,and to clarify the scientific connotation of electroacupuncture , and to provide a theoretical basis for the combination of electroacupuncture and assisted reproductive technology to improve the pregnancy rate and reduce the abortion rate of PCOS patients.

卵母细胞质量是影响多囊卵巢综合征（PCOS）辅助生殖成功率的重要因素之一，卵巢局部调控因子水通道蛋白9（AQP9）对卵母细胞的成熟、受精及以后胚胎的发育起着重要作用。众多研究和课题组前期工作表明针刺能够提高PCOS辅助生殖临床妊娠率，但具体作用机制仍不明确。本项目拟以PCOS大鼠模型为研究对象，电针为干预手段，综合应用生物信息学、分子生物学、生殖生物学等多学科交叉手段，探讨AQP9在超促排卵PCOS大鼠动情周期卵巢组织中的表达规律及AQP9对卵母细胞质量的调控作用，并观察电针对超促排卵PCOS大鼠卵母细胞质量与发展潜能的影响，从AQP9信号传导通路探讨其作用机制及作用靶点，以阐明电针助孕的科学内涵,为电针与辅助生殖技术结合提高PCOS 患者妊娠率、降低流产率的治疗策略提供理论基础。

卵母细胞质量是影响多囊卵巢综合征（PCOS）辅助生殖成功率的重要因素之一，卵巢局部调控因子水通道蛋白9（AQP9）对卵母细胞的成熟、受精及以后胚胎的发育起着重要作用。本研究以AQP9为切入点，采用分子生物技术方法、生物信息学等方法，探索AQP9对超促排卵PCOS大鼠卵母细胞质量和发育潜能的作用及机制，并探究电针对超促排卵PCOS大鼠卵母细胞质量和发育潜能的影响及机制。结果发现PCOS大鼠卵巢过量的AQP9可能通过产生氧化应激，调控Bax/Bcl2途径导致颗粒细胞凋亡，最终导致成熟卵泡减少、闭锁卵泡增多从而影响卵母细胞的发育成熟。电针对PCOS大鼠卵巢形态学，血清性激素的具有治疗作用，电针可能通过调节AQP9的基因表达来调控Bax/Bcl2途径改善颗粒细胞的凋亡情况。电针还可能通过类固醇激素生物合成、视黄醇代谢、细胞色素P450对外源性物质的代谢和PPAR信号通路影响PCOS大鼠卵母细胞质量和发育潜能，从而改善辅助生殖结局。本研究初步阐明电针治疗多囊卵巢综合征卵母细胞质量和发育潜能的作用靶点和机制，为电针与辅助生殖技术结合提高PCOS患者妊娠率、降低流产率的治疗策略提供理论基础。