线虫减数分裂cohesion复合体和HORMA蛋白相互作用分子机制的研究

The normal assembly and disaggregation of the synaptonemal complex (SC) is crucial for the precise separation of meiotic chromosomes, the assembly and dysfunction of SC are closely related to human infertility, pregnancy-induced abortions and neonatal defects (eg trisomy syndrome). The HORMA proteins and the cohesin complexes are core components of the meiosis chromosomal axis and are essential for SC assembly. It is reported that there is a close correlation between the two complexes in terms of function and localization, however, whether there is a direct interaction between them and whether they have any significance for the meiosis process remains to be elucidated. In this project, the molecular basis of the interaction between the cohesin complexes and the HORMA proteins was analyzed using Caenorhabditis elegans as the research system. The influence of the interactions between the two complexes on the meiosis chromosomal axis formation, SC assembly and dynamics, and homologous recombination were studied in vivo, and further explore the effect of post-translational modification of proteins on the interaction between complexes. Preliminary results indicate that there is a direct interaction between the two complexes, so we speculate that this interaction is critical to the assembly and process of meiosis. A series of studies will be conducted to test this hypothesis. This study will provide new insights for understanding the basis of assembly of the association complex and the regulatory mechanism of the meiosis process.

联会复合体（synaptonemal complex, SC）的正常组装和解聚对于减数分裂染色体的精确分离是至关重要的，SC的组装和功能异常与人类不育、孕期流产及新生儿缺陷（如三体综合征）等生殖健康密切相关。HORMA蛋白和cohesin复合体是减数分裂染色体轴的核心组分，是SC组装必不可少的。这两个复合体之间被报道存在着功能及定位上的密切相关性，它们之间是否存在直接相互作用有待于阐明。本项目以秀丽隐杆线虫为研究体系分析cohesin复合体和HORMA蛋白相互的分子基础，体内研究相互作用对减数分裂染色体轴的形成、SC的组装和动态性以及对同源重组进程的影响，并探索蛋白质翻译后修饰对复合体间相互作用的影响。结果表明两种复合体间存在直接相互作用，我们推测这一相互作用对SC的组装及减数分裂进程至关重要，将开展一系列的研究验证这一假设。该研究为理解SC组装的基础、减数分裂进程的调控机制提供新的认识。

联会复合体（synaptonemal complex, SC）的正常组装和解聚对于减数分裂染色体的精确分离是至关重要的，SC的组装和功能异常与人类不育、孕期流产及新生儿缺陷（如三体综合征）等生殖健康密切相关。HORMA蛋白和cohesin复合体是减数分裂染色体轴的核心组分，是SC组装必不可少的。这两个复合体之间被报道存在着功能及定位上的密切相关性，它们之间是否存在直接相互作用有待于阐明。本项目以秀丽隐杆线虫为研究体系分cohesin复合体和HORMA蛋白相互的分子基础，体内研究相互作用对减数分裂染色体轴的形成、SC的组装和动态性以及对同源重组进程的影响，并探索蛋白质翻译后修饰对复合体间相互作用的影响。结果表明两种复合体间存在直接相互作用，我们推测这一相互作用对SC的组装及减数分裂进程至关重要，将开展一系列的研究验证这一假设。该研究为理解SC组装的基础、减数分裂进程的调控机制提供新的认识。