水稻CRC2蛋白在减数分裂联会复合体形成中的分子机理研究

The synaptonemal complex (SC) is a super protein lattice that connects homologous chromosomes in meiosis I progress. This special organization is related to the meiosis processes such as homologous chromosomes pairing, synapsis, recombination,segregation, etc. Therefore,the research of the related genes during this progress will strength our knowledge about the exact mechanism of meiosis. Here,we got a novel rice mutant with asynapsis phenotype through the ionizing radiation, and we isolated this new gene using the map-based cloning method. The immunolocalization analysis found that CRC2 protein localized on the central region of the synaptonemal complex, suggesting the possibility role of CRC2 in SC formation. In addition, we found that CRC2 can interact with another reported SC central region protein CRC1, which confirmed our conclusion. However, we are still puzzled about the exact role of CRC2 in the whole meiosis process and the exact mechanism of rice SC formation . Based on our existing results, along with a serious of experimental methods, we deep investigated the function of CRC2 protein. Through our research,we hope that we can illustrate these two questions: ① the function of CRC2 in homologous pairing and recombination. ② the pathway and interactions among different proteins of rice SC. Finally, our research work will provide the new theoretical basis for the gamete development in plant, as well as in the biological hotspots of SC formation during meiosis.

联会复合体是减数分裂前期I同源染色体间由不同蛋白构成的超级复合结构，它与染色体的配对、联会、重组和分离等过程密切相关。对相关蛋白的研究有助于我们加深对减数分裂整体机制的理解。CRC2是我们前期通过辐射诱变育种以及图位克隆方法获得了一个与水稻减数分裂联会复合体形成相关的新基因。免疫荧光定位发现CRC2蛋白以线性信号定位于联会复合体的中央，表明其可能参与联会复合体的组成。此外，CRC2蛋白与另一个联会复合体中央元件蛋白CRC1互作进一步证实了这一结论，而目前对于CRC2蛋白如何影响整个减数分裂过程以及水稻联会复合体形成的精确机制仍不清楚。本研究在前期基础上，通过深入分析CRC2蛋白的功能，以期实现①阐明其在同源染色体配对和重组过程中的作用；②解析水稻联会复合体形成的网络机制；通过我们的研究，将不仅为植物配子体发育提供新的理论基础，同时也为研究减数分裂联会复合体形成这一生物学热点问题提供思路。

联会复合体是减数分裂前期I同源染色体间由不同蛋白构成的超级复合结构，它与染色体的配对、联会、重组和分离等过程密切相关。对相关蛋白的研究有助于我们加深对减数分裂整体机制的理解。CRC2是我们前期通过辐射诱变育种以及图位克隆方法获得了一个与水稻减数分裂联会复合体形成相关的新基因。免疫荧光定位发现CRC2蛋白以线性信号定位于联会复合体的中央，表明其可能参与联会复合体的组成。此外，CRC2蛋白与另一个联会复合体中央元件蛋白CRC1互作进一步证实了这一结论，而目前对于CRC2蛋白如何影响整个减数分裂过程以及水稻联会复合体形成的精确机制仍不清楚。本研究在前期基础上，通过深入分析CRC2蛋白的功能，阐明了其在同源染色体配对和重组过程中的作用；同时也解析水稻联会复合体形成的网络机制；通过克隆水稻P31comet基因，首次提出有丝分裂过程纺锤体组装监控基因P31comet参与减数分裂过程，并且认为该基因功能与DSB形成以及联会复合体装配相关；相关工作发表在PNAS、Frontier in Plant Science等国际期刊，后期工作将重点转向植物中纺锤体组装监控检验点通路基因的功能解析。