莱茵衣藻高产氢突变体氢气产量提高的分子机制研究

It has become an effective way to solve the energy crisis and environment pollution by utilizing hydrogen energy nowadays. Micro algae, as the source of hydrogen, will play an important role in sustainable production of clean energy in the future. Several ways is available to investigate and improve the hydrogen production by Chlamydomonas reinhartii, such as building mutant libraries and screening valuable mutant strains. Therefore the database of gene function will be enriched and the experimental basis will be established to improve the hydrogen production of C. reinhartii. Genome walking kit was applied in this study to localize the position of mutational genes, and verify functional genes. Molecular mechanism was investigated for the mutant strains with high hydrogen production by constructing over expression and the complementary algae strains of mutational gene. Furthermore, physiological data was added to support the finding. This study is of great significance to improve the hydrogen production by constructing genetically engineered algae strain.

氢能源的开发利用是解决能源危机及环境问题的有效途径，微藻制氢技术是未来清洁能源可持续生产的重要组成部分。通过构建莱茵衣藻突变体库、筛选突变株是挖掘调控产氢新基因并揭示基因功能的有效途径。本研究将筛选到的莱茵衣藻高产氢突变株的突变基因进行定位，并通过构建突变基因的过表达及互补藻株的方法对基因功能进行验证，同时结合生理数据测定确认基因功能，以期挖掘到衣藻产氢调控的新基因同时可以揭示突变株产氢量提高的分子机制，为利用基因工程手段构建高产氢藻株提供理论及实验基础。

氢能源的开发和利用是解决能源危机及环境问题的有效途径，微藻制氢技术是未来清洁能源可持续生产的重要组成部分。本研究筛选到一株高产氢莱茵衣藻突变株，其氢气产量提高7~8倍，其生长并未受到抑制，反而略有升高，通过转录组测序发现其突变位点位于细胞核第13号染色体上的ITN7基因第三个内含子区，然后通过构建突变基因的过表达及互补藻株的方法对基因功能进行验证，同时结合生理数据测定确认基因功能，以期阐明该位点与莱茵衣藻产氢调控的关系，揭示突变株产氢量提高的分子机制，为进一步利用基因工程手段构建高产氢藻株提供理论及实验基础，并为莱茵衣藻产氢调控网络的构建奠定基础。