智能聚合物双水相萃取与分子印迹膜联用机制及其分离纯化龙葵碱

The purpose of this topic is to construct a new green coupling separation method on the smart polymer aqueous two-phase system (SPATPS) and molecular imprinted membrane technique (MIMT). It is a safe, non-toxic, recyclable and smart system and has the properties of scheduled structure-activity, specific recognition, exclusive choice and the advantages of high efficiency. The new separation method has realized the implementation of liquid-liquid extraction with high selectivity, wide applicability and eco-friendly solid-liquid specific separation with high.recognition. The research had selected solanine as its targets and studied the phase-separation and phase-transfer mechanisms of SPATPS. The response surface methodology(RSM) was adopted in the multi-factors optimizing experiments in order to discuss the affecting mechanisms of the different factors and to establish the thermodynamic and dynamic models for separating solanine in the SPATPS systems; it also studied the mechanisms of specific separating solanine by using MIMT and discussed the rule of pressure gradient, concentration gradient and filtration time on the separation of solanine; it also studied the relationship between the structure and nature of molecular imprinted membrane and the distribution behavior, distribution mechanisms and distribution coefficient of solanine in MIMT system; it improved the distribution theory of MIMT during the separation procedures; the validity of the new method will be also tested in order to implement the green method of the solanine separation, improve the extraction efficiency and product purity, reduce production cost, and provide a new method for the separation of biologically active factors.

本课题旨在构建智能聚合物双水相与分子印迹膜联用的绿色提取新方法，即建立一种具有构效预定性、特异识别性、选择专一性的高效、安全、无毒、可循环的智能分离体系；实现具有高富集倍数、宽适用性、绿色无污染的液-液萃取，和具有高辨识度的固-液选择性分离。本课题拟选择天然产物—龙葵碱为目标物，研究智能聚合物双水相体系的分相、相转移机制；利用响应曲面多因素优化实验，探讨各因素的影响机制；建立适用于智能聚合物双水相体系中龙葵碱分配的热力学和动力学模型；研究分子印迹膜选择性分离龙葵碱的过程机理；探讨压力梯度、浓度梯度、过膜时间等因素对龙葵碱分离的影响规律；探求龙葵碱在分离体系中的分配行为、分配机制和分配系数与分子印迹膜结构和性质的关系；构建和完善分子印迹膜分离过程的分配理论。进行新方法的实效性考察，实现龙葵碱的绿色提取，提高提取效率和产品纯度，降低生产成本，为生物活性功能因子的提取提供一种新的方法。

构建了一种新型的萃取分离体系：将可循环的智能聚合物双水相萃取技术与分子印迹膜分离技术联用，实现了技术优势互补，建立了具有识别特异性、选择专一性的可循环萃取分离新体系，并应用该体系分离/纯化植物中的天然产物－龙葵碱（以龙葵中的澳洲茄边碱为代表目标物）。.①构建了智能聚合物-盐双水相体系，测定了系线数据和双节线数据，并建立了分相过程和物质在两相间分配行为的动力学和热力学模型。.②分析了澳洲茄边碱及龙葵叶中各主要成分的理化性质，筛选出适用于澳洲茄边碱萃取分离的智能聚合物双水相体系；基于温敏性聚合物的温度响应行为，实现了澳洲茄边碱的控温分级分离，解决了目前成相物质难以分离回收的问题。.③设计并合成了对澳洲茄边碱具有特异选择识别功能的多功能印迹复合膜，构建了智能聚合物双水相萃取-分子印迹膜分离纯化澳洲茄边碱体系，实现了龙葵植物原液中澳洲茄边碱的高纯度分离，解决了膜污染和澳洲茄边碱选择性不佳的问题。