手性自组装短肽加速修复创伤过程中的分子细胞机制

Here we propose to understand the molecular mechanism of chiral self assembling peptides to accelerate skin wound-repairing from the molecular, cellular and animal models. We use chiral self-assembling peptide nanomaterials, regulation of the formation of 3D-microenvironment and controlled release of growth factors, get the relationship between the self-assembling peptides molecule process with the 3D microenvironment reconstruction, and obtain the kinetic process of controlled release of growth factors; We investigate cell growth behavior and protein expression to the reconstructed microenvironment, and analyze the results of Integrin- and FAs-Facilitated Rho Signaling associated with the mechanotransduction to chiral self-assembling peptide 3-D extacelluar matrix. Then we combine SD rat skin wound repairing data, get the hypothesis and model of "3D reconstruction of microenvironment-rapid-growth factor wound repairing", and clarify the principle and approach of rapid skin wound healing. This work will clarify the mechanism of chiral self-assembling peptide acceleration the process of skin wound repairing including the interaction to the linked cells, tissues and organisms and beyond.

本课题拟从分子、细胞和动物机体模式下阐释手性自组装短肽加速皮肤创伤修复的分子细胞机制问题。采用手性自组装短肽纳米生物材料，调控三维微环境的形成和生长因子的控释，明确短肽分子自组装过程与三维微环境重塑的关系，得到生长因子控释的动力学过程；考察细胞在该重塑后微环境的生长行为和蛋白表达，以及纳米机械应力对整合素-黏着斑-Rho”信号通路的影响；结合SD大鼠皮肤创伤修复数据，建立“纳米三维微环境重塑-生长因子创伤快速修复”假说和模型，阐明其加速皮肤创伤修复原理与途径；明确手性自组装短肽纳米材料在皮肤创伤快速修复过程中，对相关细胞、组织和机体的作用机制。

本课题从分子、细胞和动物机体模式下研究了手性自组装短肽加速皮肤创伤修复的分子细胞机制问题。合成手性自组装短肽纳米生物材料，分析三维微环境的形成和生长因子的控释，短肽分子自组装过程中各影响因素与三维微环境重塑的关系，得到代表性生长因子控释的动力学过程；测试细胞重塑后微环境中的生长行为和重要蛋白表达，以及纳米纤维机械应力对整合素-黏着斑”信号通路中重要分子的影响；结合皮肤创伤修复数据，建立“手性自组装短肽创伤快速修复”学说和模型，明确加速皮肤创伤修复主要原理与重要途径；证实了手性自组装短肽纳米材料在皮肤创伤快速修复过程中，对相关细胞、组织和机体的重要作用机制。