申嗪霉素对水稻白叶枯病菌的药理学机制研究

Shenqinmycin, commercialized in 2003, is a microbic and agro- antibiotic with the whole intellectual property, high-effectivity and environment-friendly feature. Previous research results showed this antibiotic is effective to lots of important plant pathogens, including fungi and bacteria. The pharmacological mechanism of the antibiotic, however, is unclear. Xanthomonas oryzae pv. oryzae, which causes rice leaf blight, was applied as a target organism to uncover the mode of action of Shenqinmycin in this programme. Firstly, effect of the antibiotic on morphology and physiological and chemical pathways were analyzed.Then, differential proteins from the bacterium treated by the antibiotic were sparated, idendified and analysed by bioinformatics.Then, biochemical pathoways related were analyzed to confirm the results of bioinformatics.Finally, knockout mutants, complementary mutants and over-expressed mutants of the genes coding the key proteins on the physiological and chemical pathways were preferentially constructed to furtherly analyze the importance of these genes in the mode of action. Firstly,the results of this programme will be helpful to use this antibiotic scientifically in the field, elongate the commercial life and retard development of the resistance. Secondly, the results is conducive to optimize the orginal structure of Shenqinmycin and create more effective and environmental friendly fungicides with a more wide anti-pathogens spectrum.?Five papers will be released, including 3 papers published in SCI resource journals.

申嗪霉素是我国具有完全自主知识产权、高效、与环境友好的微生物源抗生素，于2003年商业化。前期研究表明，该药剂对很多植物病原真菌和细菌都有效，但是目前其对病原菌的药理学机制仍不清楚。本项目拟以引起水稻白叶枯病菌为靶标生物，（1）观察药剂作用于菌体后的形态学特征；（2）通过药剂处理后的蛋白质组学变化，经分离和鉴定，采用生物信息学分析作用于菌体细胞的可能生理生化途径；（3）生理生化测定和验证；（4）构建可能的靶标蛋白质的基因全/部分敲除、回复、过表达突变体，验证突变体的药敏性变化，旨在系统研究药剂对作物病原细菌的药理学机制。该研究成果将为申嗪霉素的科学使用、有效延长药剂使用寿命、延缓抗药性形成等奠定理论基础，科学地指导当前生产；有助于研发人员对药剂原有的化学结构进一步优化，创制出更加高效、广谱和环境相容的杀菌剂。发表论文5篇，其中SCI论文3篇。

申嗪霉素是我国具有完全自主知识产权、高效、与环境友好的微生物源抗生素，于2003年商业化。本项目以水稻白叶枯病菌为靶标生物，水稻细菌性条斑病菌和野油菜黄单胞均为申嗪霉素不敏感对照菌株作为研究材料，从形态学特征、蛋白质组学、Tn5插入突变体库、抗性菌株的诱导和潜在靶标基因的敲除、回复，系统研究了申嗪霉素对水稻白叶枯病菌的作用机制和抗性机制。