基于感光高分子表面自组装改性的超高效液相色谱固定相研究

UPLC stationary phases are of great importance to the application of UPLC in the different areas such as life science and.pharmaceutical analysis. How to avoid the disadvantages of the traditionally used silanizing agents in the surface functionalization of UPLC stationary phases to achieve high performance has drawn the most attention recently. In our project, the goal is to establish a systematic method to realize the high performance UPLC stationary phases by controllable layer-by-layer self-assembly technique. Instead of the traditional silanizing agents, photosensitive polymers will be designed and applied to the self-assembly to achieve non-covalent bonded coatings on the surfaces of UPLC stationary phases, which change to covalent bonded coatings after photocrosslinking. The properties of the obtained stationary phase coatings, as well as their applications in the chiral and mixed mode chromatographic separation will be investigated.

UPLC技术在生命科学和药物分析等领域都显示出重要的应用前景，UPLC技术的核心是基于亚2µm小粒径硅胶的色谱填料。日新月异的应用要求对UPLC固定相的表面改性制备提出了新的挑战，在制备过程中，如何能够克服传统硅烷化改性试剂的缺点，构筑具有复杂结构、高键合密度、高负载率、高选择性、高质量、高性能的UPLC色谱填料表面改性涂层成为一个倍受关注的问题。本课题旨在探讨如何利用水溶性感光高分子的层层自组装取代毒性大、污染强、易水解的硅烷化试剂，通过对UPLC色谱填料表面改性涂层制备、性能的创新，建立具有结构可控、适合手性和多种混合模式分离等应用需要的UPLC色谱填料表面改性涂层自组装构筑的系统方法，利用感光高分子独特的光、热诱导原位交联反应实现对UPLC色谱填料表面自组装涂层的共价键合和固定化，并研究所制备的UPLC色谱填料表面改性涂层的物理化学性质，探索其在手性和混合模式色谱分离等方面的应用。

UPLC技术在生命科学和药物分析等领域都显示出重要的应用前景，UPLC技术的核心是基于亚2µm小粒径硅胶的色谱填料。日新月异的应用要求对UPLC固定相的表面改性制备提出了新的挑战，在制备过程中，如何能够克服传统硅烷化改性试剂的缺点，构筑具有复杂结构、高键合密度、高负载率、高选择性、高质量、高性能的UPLC色谱填料表面改性涂层成为一个倍受关注的问题。本课题利用水溶性感光高分子的层层自组装取代毒性大、污染强、易水解的硅烷化试剂，通过对UPLC色谱填料表面改性涂层制备、性能的创新，建立了具有结构可控、适合手性和多种混合模式分离等应用需要的UPLC色谱填料表面改性涂层自组装构筑的系统方法，利用感光高分子独特的光、热诱导原位交联反应实现了对UPLC色谱填料表面自组装涂层的共价键合和固定化，并研究了所制备的UPLC色谱填料表面改性涂层的物理化学性质，探索了其在手性和混合模式色谱分离等方面的应用。