眼眶重建用骨替代品纳米羟基磷灰石及其复合材料的合成及应用研究

Hydroxyapatite(HAP),as a biologically active materials, has some defects such as brittleness, low-bending strength and its pores size directly affect the growth of the bio-tissue. According to requirements of anophthalmus reconstruction and in order to solve the above questions, porous hydroxyapatite nanocomposites materials were designed and prepared by the ways of wet chemical synthesis and the technology of control of structure and surface in this research. By decreasing the size of HAP crystalline, orderly orientation, its mechanical properties could be enhanced. Assembling of nanoparticles could prevent the transport,and decrease the potential danger in the human body.The relationship of size of pore, structure and morphology with the biocompatibility and bility of tissue growth in the materials would be studied in detail.Characterize and analyze the inteface of the materials and tissue, and get to know how HAP surface structure control the cell growth and messages transfer. In a word, HAP as a replacing orbital bones with good mechannical properties, biocompatibility and so on would be designed and systhesized.

本项目根据眼眶重建用骨替代材料的要求，针对目前羟基磷灰石义眼台存在的力学性能和传导成骨问题进行结构和组成、表面设计，采用湿法化学合成与微结构和表面调控技术相结合的方法合成多孔的羟基磷灰石复合材料。通过HAP晶体在复合材料中的纳米化、规则取向和有序排列来提高HAP生物复合材料的力学性能，通过自组装解决纳米迁移问题，利用高分子表面接枝氨基酸序列来提高复合物的生物活性和调节HAP晶体取向生长的能力，研究骨替代材料的传导成骨能力，通过细胞和动物实验明确种植体表面形貌与组织界面结合特性、对细胞选择过程及相关信号因子的影响规律，探索出骨替代材料表面拓扑结构对细胞生长和相关信号通路的控制机制，实现骨替代材料-骨组织界面有效结合的再生设计，获得骨替代材料表面纳米化设计和纳米结构控制方法，最终获得具有良好的力学相容性、生物相容性、骨传导性、骨诱导性的羟基磷灰石眼眶骨替代材料。

项目执行期内，以羟基磷灰石材料为研究背景，重点研究了羟基磷灰石颗粒的制备及修饰成型工艺及其在眼眶修复方面的应用，以功能为导向针对主要取得了如下成果：.分析了目前商用天然珊瑚义眼台材料结构组成特点；以湿法化学合成羟基磷灰石颗粒为基础上，通过一次成型的多孔羟基磷灰石制备工艺并实现材料多级孔道的构建；应用扫描电镜对材料形貌进行了表征，X射线衍射及红外光谱等表征手段对制备的材料进行了细致的组成分析；发现自制羟基磷灰石材料无杂质与商用材料组成一致，成型后的材料具有与商用材料类似的多级孔道结构;同时，对材料的制备条件进行了优化，确定了最佳配方。..项目执行以来，发表SCI论文10篇，包括Nature Energy、Adv. Mater.、和Chemical Society Reviews等国际知名期刊；申请中国发明专利2项。培养博士研究生2名，一名参与人晋升副教授。