纤毛虫-藻类共生系统的结构及共生作用机理的研究

By means of the electron microscopic structure, biochemistry and molecular biological methods, the cytoplasm structure, organelle enzyme activity, organelle isoenzyme and DNA diversity were compared in chlorellae-bearing and chlorellae-free Paramecium bursaria. It is suggested that the algae symbiont can affect host organellar function, quantity and location, stimulate the food uptake and digestive activity, restrain the functional activity of the organelle, change the gene expression and cause the change of genome structure in the P. bursaria-chlorellae system. The results will contribute to a better understanding of the interaction between cells, the structural foundation of symbiont origin, the pattern of life processes surrounding the cellular structures and functions, and the theory of ensymbiosis and cell evolution in eukaryoytes.

研究纤毛虫－藻类共生系统在建立、整合过程中宿主和内共生体的细胞超微结构，宿主细胞质酶系的特征，宿主细胞蛋白质的组成和细胞核基因组DNA的变化, 探索绿色纤毛虫共生系统的形成和作用机理，所得结果对揭示原生动物－真核生物共生系统中结构与功能的关系、作用机理，丰富和发展细胞内共生生物学的理论，有重要科学意义。