抗氧化的血红蛋白组装及其生物物理特性的研究

The project put forward a method that fabricating antioxidative hemoglobin-based assembly through layer-by-layer technique. The antioxidative hemoglobin-based assembly can not only effectively prevent the oxidation of hemoglobin, maintaining its inherent activity of the oxygen carrier, but also play the double effect of the oxygen carrier and antioxidants in the treatment of injury caused by hypoxia-ischemia. Combined with the advantages of layer-by-layer assembly technique, the structure and property of hemoglobin-based assembly can be precisely regulated by changing the template, wall composition, driving force, surface modification et al, obtaining the optimized hemoglobin-based assembly. Then the antioxidative hemoglobin-based assembly was applied into the cells and animals experiments, testing the biocompatibility and hemocompatibility of the assembly. The as-prepared antioxidative hemoglobin-based assembly would have potential applications in artificial blood, ischemia-reperfusion injury, as well as other injury caused by hypoxia-ischemia.

本项目以血红蛋白为主要组装基元，选择不同的抗氧化剂，基于层层组装技术和模板法制备一系列具有抗氧化活性的血红蛋白组装体。该组装体不仅可以有效防止血红蛋白的氧化，保持其固有的载氧活性，还可以在缺氧缺血导致的机体损伤的治疗中起到氧载体和抗氧化剂双重作用。通过改变模板、囊壁成分、组装驱动力、表面修饰等手段调控血红蛋白组装体的结构与功能。在此基础上，完成血红蛋白组装体的体外细胞实验和动物缺血再灌注模型实验，为其在人造血液、缺血再灌注损伤以及其它缺氧导致的机体损伤治疗中的应用提供重要的实验依据。

寻找安全有效地血液应急材料在应对因突发事件而引起的人员伤亡中具有重要的现实意义。基于血红蛋白的血液应急品具有高载氧性和良好的生物兼容性，已成为血液应急生物材料的首选体系。目前对该课题的研究仍面临诸多困难，例如如何增强血红蛋白的稳定性、防止血红蛋白氧化成无载氧活性的高铁血红蛋白。本项目以血红蛋白为主要组装基元，基于层层组装技术和模板法制备具有抗氧化活性的血红蛋白组装体，从而防止血红蛋白的氧化，保持其固有的载氧活性；研究了血红蛋白组装体的生物相容性、生物可降解性、抗氧化性以及细胞毒性；并初步探讨了血红蛋白组装体在氧化应激损伤治疗中的应用。