地震后儿童创伤后应激障碍与长时程增强通路相关基因的关联性研究

Post Traumatic Stress Disorder(PTSD) in children is a severe disease which would constitute a serious risk to mental health and growth in children, but its mechanism is also unsure. Post Traumatic Stress Disorder in children is very important for mental health service after disaster. Preliminary work have done to study PTSD in children after Wenchuan earthquake. In these preliminary work,we found that many gene have no association with PTSD in children after earthquake,we also found that the prevalence rate of PTSD reduced in 3 years follow-up wok, while children were growing. So we had a hypothesis that synaptic plasticity had it affect to children’s PTSD. Synaptic plasticity was considered to be a neurochemical basis of study and memory. The Long-term potentiation(LTP) pathway was central field of research among synaptic plasticity. And it ia also proved that stress can induce change in LTP and Synaptic plasticity by preliminary animal experimentation, and this supported our hypothesis. Based on this hypothesis, we will carry out the study of the predisposing genes related to the LTP pathway, which also has association with preliminary work in data analysis. In this study ,we will reveal the mechanism of children’s PTSD in order to find new ways of taking better diagnosis and therapy to this disease.

儿童创伤后应激障碍是严重危害儿童心理健康和心理发育的疾病，其确切机制尚未阐明。我国是一个地震频发的国家，地震后儿童创伤后应激障碍是一个重大的精神卫生问题。本课题组前期对汶川地震后儿童创伤后应激障碍的研究发现：多个与5羟色胺和多巴胺受体相关基因的遗传学改变与地震后儿童创伤后应激障碍并没有明确关联，而在接近疾病的自然进程的情况下地震后儿童创伤后应激障碍的时点患病率却随着患儿年龄的增长在下降。故我们推测：以长时程增强通路为主要机制的神经突触可塑性对地震后儿童创伤后应激障碍的起病可能具有重要影响。我们前期动物实验的结果亦证实了应激可以导致相应的突触可塑性及长时程增强电位的变化，该结果有力地支持了我们的研究假设。故本研究将对长时程增强通路的相关基因与地震后儿童创伤后应激障碍进行关联性研究，并结合前期工作，深入探讨地震儿童创伤后应激障碍的发病机制，为早期诊断和治疗地震后儿童创伤后应激障碍提供依据。

儿童创伤后应激障碍是严重危害儿童心理健康和心理发育的疾病，其确切机制尚未阐明。我国是一个地震频发的国家，地震后儿童创伤后应激障碍是一个重大的精神卫生问题。本研究在大样本流行病学研究的基础上，对相关的地震后儿童创伤后应激障碍患者随访的基础上，对长时程增强通路的相关基因与地震后儿童创伤后应激障碍进行关联性研究，本项研究结果发现：在大样本流行病学的调查数据基础上发现了地震后儿童PTSD的发生与地震主观经历、相关人格因素有密切关系，儿童PTSD症状的恢复与相关地震经历及地震后早期人格因素有密切关系，发现了与地震后儿童PTSD发病的易感基因的SNPs为G72基因的rs778294、S100B基因的rs2300403、DISC1基因的rs821597和rs821616，与儿童PTSD症状恢复的相关因素为：相关地震经历，地震后早期人格因素，rs778294的TT基因型和TC基因型。本项研究为重大灾害后儿童心理应激反应的诊断和治疗提供了相应的依据。