枸杞的体细胞杂交研究

Wild Lycium barbarum and Lycium ruthenicum growing in the Qinghai-Tibet Plateau will be chosen as protoplast donors, and cultivated L. barbarum in Ningxia will be chosen as recipients. Based on the development of high frequency protoplast division and plantlet regeneration, the intraspecific and interspecific somatic hybridization between wild and cultivated Lycium will be performed, and the hybridity of calli and regenerated plantlets will be identified as well as the genetic characterization and composition of hybrid plants will be analyzed. These will provide scientific references for the transferring of stress-related genetic characterization of wild plants by somatic hybridization technology, and provide useful materials for the further researches on the variety breeding of Lycium.

以生长于青藏高原地区的野生宁夏枸杞（Lycium barbarum）和黑果枸杞（Lycium ruthenicum）为供体，以宁夏地区人工种植的宁夏枸杞（L. barbarum）为受体，在建立起原生质体高频率分裂和再生植株的基础上，开展野生枸杞和栽培枸杞的种内和种间杂交研究，对杂种愈伤组织和再生植株的真实性进行鉴定，并分析杂种植株的遗传性状及组成，为通过体细胞杂交技术转移野生植物抗逆遗传性状提供科学依据，同时为枸杞品种选育提供有用材料。

黑果枸杞（Lycium ruthenicum Murr.）主要分布于青藏高原地区，是一种对极端环境高度适应的经济植物，通过细胞工程技术转移黑果枸杞的有益遗传性状不仅具有一定的经济价值，而且还具有重要的生态意义。本课题以自然生长于青藏高原地区的野生黑果枸杞（L. ruthenicum）和宁夏地区人工种植的宁夏枸杞（L. barbarum）为试验材料，建立了黑果枸杞和宁夏枸杞的组织培养再生体系和原生质体培养再生体系；通过黑果枸杞和宁夏枸杞的不对称体细胞杂交，获得再生植株，并对再生植株进行了形态和分子鉴定；克隆了黑果枸杞花青素合成相关基因LrTTG1和LrANS的cDNA全长序列，研究了这两个基因的表达及功能。该研究为枸杞资源的开发以及枸杞适应环境的机制奠定了基础。