噻森铜与枯草芽孢杆菌B-001协同增效的分子调控机制

The strategy of integrated chemical and biological control not only control the disease effectively but also reduce the use of fungicides and could be regarded as a viable strategy to control the plant disease. Currently, however, there is hardly any knowledge about the synergistic mechanisms of combined application of chemical pesticide and biopesticide. Our previous study indicated that the endophytic Bacillus subtilis B-001 had good antagonistic effect to Ralstonia solanacearum. The synergistic effect of B-001 used in conjunction with Saisentong to control tomato bacterial wilt was obviously. B-001 not only control the bacterial wilt effectively but also significantly reduce the use of Saisentong. In order to explore the synergistic mechanisms of combined application of Saisentong and Bacillus subtilis B-001, the impact of different treatment to the colonization of Bacillus subtilis in tomato will be analyzed by fluorescently label, and the differences of the level of B-001 induce tomato resistance will be analyzed by qRT-PCR. The aim of the investigation was to illustrate the collaborative mechanism of endogenous Bacillus subtilis B-001 integrated with fungicide to control the tomato bacterial wilt. Furthermore, the results would be benefit to enrich the theoretical study of biological agents used in combination pesticides to control plant disease and in order to provide a new idea to control bacterial wilt.

生物农药与化学农药协同使用既能有效控制病害又能降低化学农药的使用量，是一种十分有前景的病害防治方法，但目前关于生物农药与化学农药协同增效的分子机理尚不明确。本课题组前期研究结果表明内生枯草芽孢杆菌B-001对青枯病菌具有良好的拮抗效果，且与相容杀菌剂噻森铜协同使用防控番茄青枯病具有明显的增效效果，不仅有效的控制了青枯病的发生而且显著降低了噻森铜的使用量。本研究拟采用荧光标记手段分析菌药协同处理对枯草芽孢杆菌在番茄体内定殖动态的影响，并利用qRT-PCR等技术分析噻森铜存在的情况下枯草芽孢杆菌诱导番茄抗病性水平的差异，从而探索噻森铜与枯草芽孢杆菌协同防控番茄青枯病的增效调控机制。研究结果对于深入了解生物农药与化学农药协同防控植物病害的增效机制具有重要意义，有助于丰富菌药联合防治植物病害的理论研究，从而为番茄青枯病田间防治提供新思路。

生物农药与化学农药协同使用进行有害生物综合防控的策略既能稳定有效地控制病虫害又能减少化学农药的使用量，是一种十分有前景的有害生物综合治理手段，目前，关于生物农药与化学农药协同使用防治植物病害的方法体系尚未完全建立，协同增效机理研究相对较少。. 本课题测定了枯草芽孢杆菌B-001与噻森铜协同防控番茄及辣椒青枯病的田间防效，建立了枯草芽孢杆菌与化学农药协同使用技术方法，构建了枯草芽孢杆菌B-001 GFP标 记的质粒pAD43-25，获得了枯草芽孢杆菌B-001的工程菌株，目前正在进行GFP标记菌株的定殖动态研究。我们的结果表明枯草芽孢杆菌与相容杀菌剂协同使用防控植物病害是可行的，本研究有助于深入阐明菌药协同协同防控增效机理，为植物病害绿色防控提供了一条行之有效的途径，应用前景广阔。