补肾抗衰方滋腻之性对衰老大鼠消化吸收功能的影响研究

The problem of aging population make study on aging and anti-aging a medical hot. Traditional Chinese medicine has rich theory and practice experience in the aging occurrence and anti-aging method. In Chinese medicine the anti-aging method of replenishing vital essence to tonify kidney is followed with interest. It is a disadvantage that the tonic has the nature of greasy which could be an influnce on stomach.Now there is no research how the nature of greasy influnce the anti-anging function of tonifying kidney formulas.In this study to propose the hypothesis that the greasy nature impede the gastrointestinal function firstly.Then it will observed and compared that what intervention effection of the four formulas with invigorating kidney , Zuogui Pill, Yougui Pill, Liuweidihuang Pill and Jinkuishenqi Pill which has the function of increasing kidney yin,warming kidney yang,invigorating kidney-essence and reinforcing kidney qi respectively, have on subacute aging model. To approach the anti-aging mechanism that different compatibilities and the methods of invigorating yin, yang, essence and qi have initially. And primitively to approach aging state of the functions in stomach intestine of subacute aging model and internal relationship between the greasy of tonify herb with the function of stomach intestine for finding the aging character in the stomach intestine of subacute aging model. By this to test and verify this hypothesis. It can not only to provide new observation indexes, and to provide reliable experimental evidence for the clinical application of different compatibility between reinforcing and reducing, but also to provide investing thinking of exploring the greasy nature in troditional Chinese medicine.

人口老龄化问题使衰老及抗衰老研究成为医学热点，中医学对此有丰富的理论和实践经验，其中补肾抗衰中药复方尤受关注。但补益药毕竟有滋腻碍胃之弊，其滋腻之性对补肾抗衰方的抗衰作用影响尚无相关研究。本课题首先提出了补益药滋腻之性有碍胃肠功能假说，然后以四种补肾抗衰经典方剂左归丸、右归丸、六味地黄丸和金匮肾气丸为观察对象，因为四方的不同配伍方式可以对脾胃运化产生不同影响，所以通过观察和比较四种补泻不同配伍补肾抗衰经典方剂对亚急性衰老模型消化系统功能的干预效果，初步探索亚急性衰老模型胃肠功能老化特征及补益中药滋腻之弊与胃肠功能的内在联系，可以验证补益药滋腻之性有碍胃肠功能假说，同时还可通过对“纯补无泻”和“补中寓泻”的抗衰机制差异的分析，进一步对假说进行分析验证，这既可以为补泻不同配伍方式的临床应用提供可靠的实验依据，又能为补益中药滋腻之性的探索提供研究思路，还能为抗衰老新药研究提供新的观察指标。

中医学善用补肾方抗衰延年，随着老龄化社会的到来，人们对中医药抗衰老的方法关注度逐年升高，甚至常自行进补补肾方药来延年益寿。但补益药尤其一些补肾经方的滋腻碍胃之弊却不为更多的人们所熟知，其滋腻之性对补肾抗衰方的抗衰作用影响如何亦无人关注。本项目对此进行了初步研究，采用D-半乳糖400mg/kg的造模剂量复制亚急性衰老模型，因为我们研究发现在此剂量下复制出的模型老化相对更为全面和稳定，肠组织的老化状态在衰老基因的蛋白表达方面尤其明显，肠组织的抗氧化能力变化不显著，但蠕动功能方面呈现相对减弱的趋势，适合于验证我们提出的“补益中药所谓滋腻碍胃之弊，可能与胃肠动力有一定的相关性”的科研假说。并且我们发现模型大鼠胰腺的微观结构老化相对于其它组织的老化程度要更为明显，说明亚急性衰老大鼠消化系统的老化性改变在胰腺的功能上可能会有更明显的特征性变化。我们在复制衰老模型的同时又给予具有不同配伍特点的四种补肾抗衰经典方剂进行干预，从微观改变来看，胰腺在模型组和给药组中的改变相较于正常组来说都非常明显，说明所谓滋腻碍胃之弊不但与胃肠动力有一定的关系，与胰腺的功能很可能存在更大的联系；纯补无泻的左归丸组和右归丸组在提高免疫功能、抗氧化能力和抗非酶糖基化能力以及改善衰老相关基因表达方面更具有优势，但是对于胃动素、肠道cajal细胞的影响效果是补中寓泻的六味地黄丸和金匮肾气丸效果更明显，尤其是六味地黄丸，在胃动素的改善上效果明显优于其它给药组，说明补益药滋腻之性与胃肠动力的变化存在相当明显的关系；但是从电镜下的微观结构的老化性变化来看，无论是模型组，还是给药组，胰腺的老化性改变都比其它组织的老化性改变明显，下一步的研究中，对于胰腺衰老和补益药滋腻碍胃之弊的相关性应作为重点研究部分深入探索。