理脾复方制剂对幼龄厌食大鼠脑肠肽-食欲中枢信号通路的机制研究

Anorexia is a common and frequently-occurring disease at the age of 1-6. And it Is the important reason leading to the malnutrition in children and affecting children's growth and development.At present, western medicine mainly for digestion, probiotics, trace elements and vitamins treatment to give priority to.Compound preparations of recuperating spleen and stomach are Chinese medicine experience in the therapy of anorexia,which is established by jianye huang  who are the word famous TCM doctor and it’s clinical curative effect is distinct.This topic is proposed to Investigate whether Compound preparations of recuperating spleen and stomach can affect the Brain gut peptide -appetite of central nervous system abnormalities and it’s mechanism for the treatment of juvenile anorexia rats using modern technology such as molecular biology.The research results will help to further reveal the medical principle of traditional Chinese medicine for the prevention and treatment of infantile anorexia, supplement and improvement of traditional Chinese medicine Compound preparations of recuperating spleen and stomach basic research for the treatment of infantile anorexia mechanism.

厌食是1－6岁小儿常见和多发病，是导致儿童营养不良，影响儿童生长发育的重要原因。目前西医主要以助消化、微生态制剂、微量元素及维生素等治疗为主。理脾复方制剂是国家级名老中医黄建业创立治疗厌食的经验方，临床疗效显著，本课题拟运用分子生物学等现代技术，探讨理脾复方制剂是否可以影响脑肠肽-食欲中枢系统的异常，以及探明理脾复方制剂治疗幼龄厌食大鼠的机制。研究结果将有助于更进一步揭示传统中医药对于防治小儿厌食的医学原理，补充和完善传统中医药复方理脾复方制剂治疗小儿厌食机制的基础研究。

小儿厌食的发生与胃肠动力和胃肠激素不足、微量元素缺乏相关，与中枢神经系统、胃肠道内脑肠肽促食因子NPY分泌不足、或抑制食欲LP分泌过多、CCK等表达紊乱相关。理脾复方制剂是名老中医治疗厌食经验方，疗效显著，为探明理脾复方制剂对幼龄厌食大鼠脑肠肽-食欲中枢机制的影响及其治疗厌食作用机制，课题组运用现代分子生物技术，采用PCR、WB等技术，完成理脾复方制剂对幼龄厌食大鼠胃窦、结肠、下丘脑组织以及幼龄大鼠胃窦与结肠平滑肌细胞、下丘脑神经元细胞CCK、LP、NPY因子表达影响研究。研究结果：（1）厌食大鼠胃肠动力与消化液分泌不足、肝胃组织结构病理变化是大鼠发生厌食的病理基础；（2）厌食大鼠胃窦、结肠、下丘脑组织脑肠肽LP、NPY、CCK-8表达异常，是脑肠肽-食欲中枢代谢紊乱的病理基础；（3）理脾复方制剂增强胃肠动力与消化液分泌，修复受损胃组织，调控厌食大鼠胃窦、结肠、下丘脑组织脑肠肽LP、NPY、CCK-8表达，可能是其治疗厌食的作用途径、机理与靶点；（4）10%理脾复方制剂含药血清对大鼠胃窦、结肠平滑肌细胞、下丘脑神经元细胞有增殖影响，能调控其细胞内脑肠肽LP、NPY、CCK-8因子表达，可能是其作用靶点。（5）拓展微量元素锌治疗厌食，调控厌食大鼠胃窦、结肠、下丘脑组织脑肠肽LP、NPY、CCK-8表达新途径。（6）简化或改良GASMC、CSMCs原代细胞培养法，细胞成活率高，培养时间缩短、成本减少，具有创新性、实用性。研究证实厌食大鼠存在脑肠肽-食欲中枢代谢紊乱病理基础，理脾复方制剂对厌食大鼠下丘脑-食欲中枢信号通路调控影响可能是其治疗厌食疗效机制与靶点所在，其补充和完善理脾复方制剂疗效机制的基础理论，具有重要的科学意义与临床价值，为厌食治疗提供新途径、新思路、新靶点，值得推广。课题组发表论文13篇（核心4篇），培养研究生4名，获成果奖励1项。