探讨白云岩喀斯特地区植物传粉系统种内表型多样性及适应性机制

Pollinator-mediated selection on the evolution of pollination system is considered to be one of the important hypothesis in the evolution and ecology of botany. But the researches on evolutionary processes and mechanisms are still disputed.Applying new technology and looking for new examples are key processes to solve the problem. This project aims to explore the evolutionary mechanism of pollination system in dolomite Karst area. We will use the current new technology about relative study fields. We take experiments on the world heritage, Shibing Karst area, which has original habitat and the unique geological features. The area can be divided into many small habitats. Different habitats are distributed with different pollination functional groups. They also differ in temperature and humidity environment. All above provide a better environment to study pollination system diversity. We plan to carry out following experiments on the pollination system evolution in the region: analyse ecological factors, quantitatively analyse and comparatively observe on floral traits, take out cross-transplanting experiments, measure reproductive mode and gene flow. Therein, we can make out the selective agents and the patterns of interaction between the pollination system and environment. This study was the first time to carry out the animal and plant interactions in dolomite Karst area, which is expected to provide more novel and unique evidences for the evolutionary model of the pollination system, and the biodiversity mechanism study in the dolomite Karst area. It has important exploring significance on understanding of the biodiversity protection in depth.

传粉者介导的传粉系统演化是植物进化生态领域重要的假说之一。但传粉系统演化过程和机制研究仍然存在争议。目前解决争论的关键途径包括寻找新例子和使用新技术等。本项目拟采用相关领域的新技术，探讨白云岩喀斯特地区种内传粉系统演化机制。重点选择生境原始、地质地貌独特的世界遗产地施秉地区开展。该区域小生境繁多，不同生境下具有不同的传粉功能群，且存在温度和湿度等气候环境的差异，这些都为研究传粉系统的多样性提供了良好的环境。计划通过生态因子的对比观察、花部特征的定量分析、交叉移栽实验、繁殖方式和基因流的测量等实验，揭示该区域内传粉系统演化的选择动力和模式。本研究的开展，在白云岩喀斯特地区动植物互作方面尚属首次，将有望为传粉系统演化的模式提供更多新证据，为研究白云岩喀斯特生物多样性机制，进一步认识生物多样性的保护具有探索意义。

世界自然遗产地施秉云台山是世界上保存最完好的白云岩喀斯特地貌，具有较高的生物多样性。针对该地区传粉系统存在种内多态的特殊现象，围绕传粉系统的环境互作模式，环境因子选择作用大小、遗传分化等问题，开展了传粉多态性的调查、交配系统、基因流和遗传多样性的对比，并对白云岩和石灰岩喀斯特传粉差异进行了分析，总结了施秉白云岩喀斯特种内传粉系统分化的特征类型，揭示了日本蛇根草、青篱柴等物种的种内传粉系统多态性的适应特征、遗传多样性及种群分化动力，并对白云岩喀斯特和石灰岩喀斯特传粉系统的差异进行了总结，发掘了施秉白云岩喀斯特的生物多样性价值。本研究揭示了白云岩喀斯特地区种内传粉多态的适应模式，丰富了植物进化生物学的相关理论，并为白云岩喀斯特生物多样性特有性提供了证据，对于相关植物资源的保护和开发利用具有意义。项目共发表论文17篇，其中SCI论文2篇，EI论文1篇，核心期刊13篇，其中第一标注7篇；项目获得贵州省人民政府授予的自然科学奖三等奖1项；共申请专利2项，获批1项；培养硕士研究生8人，其中两人已获硕士学位。