CrMSLP基因在柑橘花粉外壁发育中的功能解析

Seedlessness is an important label for citrus fruit with superior quality. The abnormality of pollen exine will cause male sterility, and result in seedless citrus fruit formation. Our previous study had identified a male sterile 2-like protein (CrMSLP) gene by cDNA microarray and RNA-seq from a seedless Ponkan mandarin. And two transcripts of CrMSLP (called CrMSLP-A and CrMSLP-B) were obtained. In this project, the functional mechanisms of the two transcripts will be studied thoroughly. The expression pattern of CrMSLP will be investigated. Complementation assay will be conducted by over-expression of CrMSLP-A and CrMSLP-B in Arabidopsis ms2 mutant. Then the over-expression and RNAi silencing of CrMSLP assays will performed in precocious trifoliate orange (Poncirus trifoliata [L.] Raf) which was a citrus relative with very short juvenile period. Expected results of this project will elucidate the functional mechanisms of the CrMSLP gene during pollen exine development in citrus, which have important theoretical and practical value for male sterility-related genes exploration and seedless citrus genetic improvement in the future.

果实无核是柑橘品质优良的重要标志。柑橘花粉外壁发育异常会造成雄性不育，从而产生无核果实。申请人前期利用无核椪柑及普通椪柑为试材，通过基因芯片技术和RNA高通量测序筛选到一个雄性不育相关基因（male sterile 2-like protein，CrMSLP），并发现该基因存在两个转录本（暂称为CrMSLP-A和CrMSLP-B）。本申请项目拟解析这两个转录本在柑橘花粉外壁发育中的功能。通过分析基因的表达模式，超量表达互补拟南芥突变体以及在短童期的早实枳中干涉目的基因，解析CrMSLP基因的功能。本项目的实施可望深入理解CrMSLP基因在柑橘花粉外壁发育中的功能和作用机制，对柑橘雄性不育基因发掘和果实无核分子改良具有重要的理论意义和实际应用价值。