用S区变异的HBV基因免疫逆转HBV转基因小鼠免疫耐性

There are 300 millions of HBV carriers worldwide and 120 millions are in our country. HBV infection is a major threaten to human health and it is an important problem to be resolved. It is considered that HBV gene mutation and the immunological tolerance to the HBV antigen is the major cause of chronic HBVinfection and the bad treatment results. Thus, it is very important to study the gene mutation of HBV and to reverse the immunological tolerance to the HBV antigen. In this study, we construct the plasmid pCMVHB-S2. S+145R. Restriction enzyme analysis, sequencing and cell transfection of the plasmid were done. The results showed that HBsAg was expressed in the transfected cells. HBVS genome with 145Arg mutation was constructed and the transgenic mice carrying the muted HBV genome was successfully established. Genic immune to reverse the immunological tolerance to the HBV antigen was studied. The results showed that the muted HBV genome was inserted into the mice genome and the target gene was expressed in the transgenic mice. pCMVHB-S2. S+145R immunization can cause a special immune response to the target antigen in the C57BL/6 mice and the transgenic mice. It is the experimental evidence to reverse the immunological tolerance to the HBV antigen by genic immune.

机体对HBV的免疫耐性和HBV基因变异是HBV广泛传播、持续感染及防治不佳的主因之一。我且压菇℉BVS基因145Arg变异质粒和建成对人HBV抗原耐受的转基因小鼠模型。拟构建全基因的HBVS基因145Arg变异质粒和建立对其耐受的转基因小鼠模型。用以上变异质粒对上述转基因小鼠行基因免疫，探索逆转宿主免疫耐性的新途径和机理，为有效防治慢性HBV感染提供砺垡谰荨