基于药效关联的五脉绿绒蒿质量评价模式的研究

Standardization on tibetan medicine concerns its securty,accountablity and effectiveness.In this project,According to the quality standard of Chinese Pharmacopoeia, the quality standard of Meconopsis quintulinervia will be preliminary developed from the following aspects---features of morphology, powder microscopic characteristics, the content of water, total ash, extract.The HPLC fingerprints of Meconopsis quintulineria will be developed with the method of high-performance liquid chromatograph, Computer Aided Similarity Evaluation System, principal component analysis, hierarchical cluster analysis and discriminate analysis were applied to study HPLC fingerprint and chemical pattern reorganization. Volatile oil chromatographic fingerprints of Meconopsis quintulineria were acquired by GC-MS technology, and potential biomarkers were filtered by orthogonal projection latent structure (OPLS). To study the acute toxicity of the ethanol extracts from the different regions of Meconopsis quintulinervia, the ethanol extracts will be prepared from different regions of Meconopsis quintulinervia and determined the content of it. Poisoning condition and death condition of the mice intragastriced with ethanol extracts will be observed within fourteen days, and modified Karber's method be used to calculate LD50 and 95% confidence. To study anti-inflammatory and analgesic effect of Meconopsis quintulinervia extract, the anti-inflammatory effect will be tested by xylene caused mice ear edema model and increase of permeability of mice capillary caused by ethanoic acid, and analgesic effect in mice will be observed by torsion and hot-plate methods.This project will provide a scientific basis for establishing rational Tibetan medicine quality standard and safe, effective using in clinical practice.

藏药质量的规范化直接关系到藏药的安全性、有效性和质量控制性。本项目在我们前期工作观察到不同地区五脉绿绒蒿化学成分存在明显差异的基础上，拟对青藏高原重要藏药材五脉绿绒蒿不同产地的性状、显微等常规质量标准的研究，观察观察形态、结构的差异；采用高效液相色谱法建立五脉绿绒蒿的HPLC指纹图谱，结合相似度评价软件、主成分分析、聚类分析对指纹图谱进行模式识别研究；采用GC-MS建立五脉绿绒蒿挥发油化学指纹图谱，结合化学计量学中的OPLS法，从指纹图谱中筛选标志化学成分；观察醇提物对小鼠的急性毒性；采用现代药理学方法，对不同组分进行抗炎、镇痛的功效进行研究，将成分的含量与药效结合起来，建立基于药效学的五脉绿绒蒿质量评价模式，以期探寻藏药质量评价的新策略。

藏药质量的规范化直接关系到藏药的安全性、有效性和质量控制性。本项目在我们前期工作观察到不同地区五脉绿绒蒿化学成分存在明显差异的基础上,对青藏高原重要藏药材五脉绿绒蒿不同产地的性状、显微鉴别、浸出物的测定等常规质量标准的研究，观察形态、结构的差异；对五脉绿绒蒿药材进行了化学成分的分离和鉴定，在此基础上，采用高效液相色谱法建立五脉绿绒蒿的HPLC指纹图谱，结合相似度评价软件、主成分分析、聚类分析对指纹图谱进行识别研究；对五脉绿绒蒿挥发性部位的提取进行了筛选和优化，建立了超临界二氧化碳萃取的最佳提取工艺，对分布于青藏高原的16个产地的五脉绿绒蒿药材挥发性部位进行提取，采用GC-MS建立五脉绿绒蒿挥发油化学指纹图谱，结合化学计量学中的OPLS法，从指纹图谱中筛选标志化学成分；观察醇提物对小鼠的急性毒性，确定该药材为低毒性药材；采用现代药理学方法，对不同产地、不同组分包括总生物碱、总黄酮及总挥发油进行抗炎、镇痛的功效进行研究，将成分的含量与药效结合起来，建立基于药效学的五脉绿绒蒿质量评价模式，以期探寻藏药质量评价的新策略。