XBP1 在椎间盘髓核细胞中的抗凋亡作用及机制研究

Intervertebral disc degeneration is a core issue in the study of spinal degenerative diseases. Researchers can use molecular biology techniques to improve and rebuild the degeneration of intervertebral disc tissue . For degenerative disc tissue reconstruction process is the most important seed cells 'nucleus pulposus cells' function cell transplantation can be taken in the way. How to improve the anti-apoptotic effect of transplanted cells is the key to the relationship between the efficacy and prognosis. X-box binding protein 1( XBP1) is XBR1 is one of important endoplasmic reticulum stress(ERS) signal regulators. XBP1 can regulate chaperone synthesis, endoplasmic reticulum-associated protein degradation, and promote cell survival. ERS-induced apoptosis mechanism nucleus / survival is uncertain. XBP1 whether to participate in the nucleus of apoptosis / survival mediated by ERS and play an important role. Further research is needed in this area to confirm the molecular biology. We believe in the nucleus pulposus cell transplantation transplant recipient microenvironment, XBP1 play a key role. And promote the survival of nucleus pulposus cells mediated by ERS. The issue to be adopted XBP1 expression in the nucleus pulposus cells, explore XBP1 anti-apoptotic ability of the nucleus pulposus cells and to clarify the influence of the molecular mechanism. This study explored XBP1 anti-apoptotic ability of the nucleus pulposus cells and to clarify the impact of its molecular mechanism of nucleus pulposus cells provide protection programs to improve the activity and biological effects of nucleus pulposus cells after transplantation.

椎间盘退变是脊柱退行性疾病的核心问题，可以通过分子生物学技术去改善和重建退变的椎间盘组织。对于退变椎间盘组织重建过程中最重要的种子细胞“髓核细胞”可以采取功能细胞移植的方式，如何提高移植细胞抗凋亡的作用是关系到疗效和预后的关键。XBR1是重要的内质网氧化应激反应的关键信号调控因子，XBP1s可调控分子伴侣的合成和内质网相关蛋白的降解，促进细胞的生存。ERS诱导髓核细胞凋亡/存活的机制尚未明确，XBP1是否能够通过介导ERS参与髓核细胞的凋亡/存活并发挥重要的作用，需要进一步的分子生物学研究去证实。我们认为在椎间盘髓核细胞移植后移植受体微环境中，XBP1发挥关键性的作用，通过介导ERS促进髓核细胞的存活。本课题拟通过在椎间盘髓核细胞中表达XBP1，探究XBP1对髓核细胞抗凋亡能力的影响并阐明其分子机制，以对髓核细胞提供保护方案，提高移植后髓核细胞的活性及生物学效应。

髓核细胞移植是治疗椎间盘退变性疾病的一种新尝试，髓核细胞移植成功的关键是种子细胞的抗凋亡能力。内质网应激介导的凋亡通路是髓核细胞凋亡的主要途径，XBP1是ERS的关键信号调控因子。本课题通过在髓核细胞中表达XBP1，通过体外细胞学实验和动物体内实验，证实了XBP1对髓核细胞抗凋亡能力的影响并初步阐明了其分子机制，提高了髓核细胞存活率，为髓核细胞的保护提供了新方案。