基于极性散射的超分辨三维全内反射显微成像方法研究

Based on the national large scale scientific infrastructure "space environment simulation and research infrastructure", aiming at solution of insufficient resolution and three-dimensional imaging difficulty for optical microscopic imaging of subcellular structure closing to cytomembrane in the research of space radiation-induced bystander effects, super-resolution three-dimention total internal microscopy based on polarized scattering would be carried out. The polarized scattering under illumination of single wave vector evanescent field in different directions and different autocorrelation cumulants of neighboring observation points relating to the symmetrical specimen structure are utilized to compress the system equivalent point spread function, significantly, which would realize super-resolution microscopic imaging. Depth-adjustable evanescent field with high wave vector is established, and combined with inverse scattering algorithm, three-dimensional imaging would be realized. Expected spatial resolution (horizontal and axial): better than 50nm; evanescent field depth adjustable range: 800nm. The project would provide scientific analysis means for space radiation effect research, proton radiation precision medicine and so on.

依托所在单位承担国家重大科技基础设施“空间环境地面模拟装置”建设任务，针对细胞膜附近亚细胞结构空间辐射旁效应光学显微观测横向分辨力不足及难以进行三维成像的问题，拟开展基于极性散射的超分辨三维全内反射显微方法研究，首先利用样品结构不对称性对不同方向单波矢倏逝场激励产生极性散射和相邻观测点自相关累积量差异，显著压缩系统有效点扩展函数，实现横向超分辨成像；其次，构建了深度可调的高波矢倏逝场，并与散射逆运算算法相结合，实现三维成像。预期空间分辨力（横向及轴向）优于50nm，成像深度：800nm。项目成果为空间辐射效应研究、质子辐射精准医疗等提供科学分析手段。

针对细胞膜附近亚细胞结构光学显微观测横向分辨力不足及难以进行三维成像的问题，开展基于极性散射的超分辨三维全内反射显微方法研究，首先利用样品结构不对称性对不同方向单波矢倏逝场激励产生极性散射和相邻观测点自相关累积量差异，显著压缩系统有效点扩展函数，实现横向超分辨成像；其次，构建了深度可调的高波矢倏逝场，并与散射逆运算算法相结合，实现三维成像。横向分辨力：48 nm，轴向显示分辨率：25 nm，成像深度：800 nm。项目成果为空间辐射效应研究、质子辐射精准医疗等提供科学分析手段。