基于群体感应系统的好氧硝化颗粒污泥形成机制及调控策略

Biological nitrogen removal process, with aerobic nitrifying granules as the core technology, has become an important developing direction of environmental biotechnology. With the aerobic nitrifying granules as the objects, the mechanisms of quorum sensing (QS) have been revealed during the aerobic nitrifying granules formation. The regulation strategy by immobilization of QS signal molecules was proposed. The main contents of this project include: (1) Analyzing the variation pattern and mechanism of QS signal molecules during formation of aerobic nitrifying granules by analytical chemistry and omics methods; (2) Improvement and its mechanism of aerobic nitrifying granules formation by adding the QS signal molecules; (3) Development of the formation and regulation strategy of aerobic nitrifying granules based on the QS signal molecule immobilization..This study belongs to the crossing field of environmental engineering, analytical chemistry and biological engineering. The significances of this project are as follows: (1) Explaining variation pattern and microbial physiology mechanism of intraspecific and interspecific QS signal molecules during the formation of aerobic nitrifying granules, providing the theoretical supports for the microbial regulation of aerobic nitrifying granules system; (2) Regulating formation and operation of the aerobic nitrifying granules by the chitosan magnetic nanoparticles immobilized by intraspecific and interspecific signal molecule, providing the technical supports for the field application of aerobic nitrifying granules.

以好氧硝化颗粒污泥为核心的生物脱氮工艺已成为环境生物技术领域的重要发展方向。本立项以好氧硝化颗粒污泥为对象，从群体感应系统入手，揭示其在颗粒污泥形成过程中的作用机制，并提出以固定化信号分子为手段的调控方法，内容包括：(1) 采用分析化学及组学手段解析群体感应信号分子在好氧硝化颗粒污泥形成中的变化规律及作用机制；(2) 投加群体感应信号分子对好氧硝化颗粒污泥形成的促进作用及机制；(3) 研发基于群体感应信号分子固定化的好氧硝化颗粒污泥培养方法及调控策略。.本研究属于环境工程、分析化学及生物工程的交叉领域，其意义在于：(1) 阐释种内和种间信号分子在好氧硝化颗粒污泥形成过程中的变化规律及微生物生理学机制，为好氧硝化颗粒污泥系统的微生物调控提供理论支持；(2) 利用固定化种内和种间群体感应信号分子的壳聚糖磁性纳米颗粒调控好氧硝化颗粒污泥的形成及运行，为好氧硝化颗粒污泥的实际工程应用提供技术支持。

以好氧硝化颗粒污泥为核心的生物脱氮工艺已成为环境生物技术领域的重要发展方向。本立项以好氧硝化颗粒污泥为对象，从群体感应系统入手，揭示其在颗粒污泥形成过程中的作用机制，并提出调控方法，目前取得的主要研究成果包括：1）好氧颗粒污泥系统稳定运行过程中进水有机物浓度对微生物群落结构变化的影响；2）好氧硝化颗粒污泥系统中 EPS主要产生菌群与群体感应信号分子（AHL）之间的影响关系；3）确定了信号分子（AHL）的类型与颗粒污泥形成之间的关系。. 本研究意义在于阐释种内和种间信号分子在好氧硝化颗粒污泥形成过程中的变化规律及微生物生理学机制，为好氧硝化颗粒污泥系统的微生物调控提供理论支持。