用丙氨酰-谷氨酰胺和维生素E调控罗非鱼冷应激代谢的分子机制

Fish metabolism and other functions are influenced by cold stress, even resulting in death. Alanyl-glutamine dipeptide (AGD) and vitamin E (VE) can promote the metabolism, immunity and antioxidation of fish. Combined application of AGD and VE can depress the stress of fish and improve the capacity of exist, development and growth. However, their working mechanism has not been understood. The present project is to study the molecular mechanism of the metabolism of tilapia in cold stress through different levels using cross subjects. Cultivate the groups of tilapia juveniles in low temperature and feed assorted feed with different AGD and VE additions for 8 weeks. Sampling fish samples for determining the values of nutrition, physiology, biochemistry and molecule. Compare the effects of low temperature and different AGD and VE additions on the metabolism of the juveniles. Understand the relationship of the metabolism of fish in cold stress and the control using AGD and VE additions. Find the optimum dose of the mixture of AGD and VE for control the metabolism of fish in cold stress. Analyze the target organs, genes and the effects of different AGD and VE additions. Understand the pathway and molecular mechanism of AGD and VE cooperation. The purpose of the project is to provide the basis of control the metabolism of fish in cold stress and to protect fish from cold. The study is not only for theory but also for application.

冷应激影响鱼的代谢及其他功能，乃至伤亡。丙氨酰-谷氨酰胺（AGD）和维生素E（VE）能促进机体代谢、免疫和抗氧化，降低应激反应，提高生存和生长发育能力，但机理未明。 本项目在前期研究的基础上，用交叉学科，从基因、分子、组织器官和整体水平，多层次研究用AGD和VE调控南方重点养殖的罗非鱼冷应激代谢的分子机制。分别用添加不同剂量AGD和VE的饲料、对照组饲料，投喂低温养殖的各组罗非鱼稚鱼8周，取样测定各组鱼营养、生理、生化、分子和基因指标变化，比较低温对鱼代谢的影响及用不同剂量AGD和VE调控其代谢的效果，理解鱼冷应激代谢与添加AGD和VE调控的关系；筛选AGD和VE协同调控鱼冷应激代谢的最适剂量；分析AGD和VE协同作用的主要靶器官、靶基因及其对不同剂量AGD和VE的效应，理解AGD和VE在鱼体协同调节的途径和分子机制。为应用微营养素调控养殖鱼冷应激代谢和保护鱼类提供基础。

按计划研究“用丙氨酰-谷氨酰胺(AGD)和维生素E(VE)调控罗非鱼冷应激代谢的分子机制”。因代谢和调控均与pH相关，经报告后，增加研究“用AGD和VE调控酸化pH对鱼代谢的分子机制”。. 已按计划完成了全部实验，达到了预定目标，发现用丙氨酰-谷氨酰胺(AGD)和维生素E(VE)调控罗非鱼的冷应激代谢、GPx和PPARa基因的效果显著(P<0.05)；用AGD与VE调控酸化pH对鱼代谢、GPx和PPARa基因影响的效果显著(P<0.05)；通过初审的国家发明专利2项；培养博士生和硕士生5名，其中2人已毕业并获得学位；参加国内外学术会议16人次，其中国际特邀主题报告2次；已发表学术论文18篇，其中SCI论文4篇；正在写作SCI论文3篇，将标注此项目资助和继续报告。