JH信号诱导二化螟Vg基因表达的转录因子鉴定及功能分析

Juvenile hormone (JH) signaling pathway plays an essential role in insect reproduction and development through regulating vitellogenin (Vg) expression. However, the mechanisms of how Vg expression is regulated by JH remain unclear, identification of specific transcription factors is the premise of revealing the transcription activation mechanism. Here, by applying Chilo suppressalis as model, our project will identify the critical transcription factors and further explain the molecular mechanisms underlying JH-induced transcription of Vg gene in C. suppressalis by methods including double luciferase reporter system, DNA Pull-down, EMSA and RNAi techniques. Our study would promote the interpretation on the molecular mechanisms of CsVg induction resulting from JH stimulation, which would further provide strong theoretical support for the establishment of biological pest control.

卵黄原蛋白（Vg）是昆虫卵黄发生的关键蛋白，与昆虫的生殖和发育密切相关。前人研究表明Vg基因的表达受保幼激素（JH）信号调控。然而，JH信号激活Vg基因表达的转录机制并不清楚，鉴定JH诱导Vg基因表达的转录因子是揭示其转录机制的前提。因此，本项目拟以二化螟为对象，采用双荧光素酶报告系统，DNA Pull-down，凝胶迁移和RNAi等技术研究JH诱导Vg基因表达的关键转录因子；并进一步探究JH信号调控二化螟Vg基因转录的分子机制。本项目的实施对阐明昆虫Vg基因的转录调控机制具有重要意义，同时为制定二化螟绿色防控策略提供理论依据。

卵黄原蛋白（Vg）是昆虫卵黄发生的关键蛋白，与昆虫的生殖和发育密切相关，这一过程受到昆虫保幼激素信号（JH）调控。本项目通过比较转录组学、RNAi及双荧光素酶报告系统等技术分析发现二化螟保幼激素信号通路中关键转录因子Kr-h1参与调控二化螟卵黄形成及卵巢发育。进一步研究发现，miRNA-1579通过靶向Kr-h1基因调控该基因表达，最终实现对卵黄形成的调节。此外，研究发现脂酰辅酶A还原酶FAR家族基因对二化螟卵黄形成发挥重要作用。本项目的实施对阐明昆虫生殖的转录调控机制具有重要意义，同时为制定二化螟绿色防控策略提供理论依据。