根特异性表达植物生长素和细胞分裂素对甘草根部形态及有效药物成分的影响

Licorice is not only an important bulk herbs, and it is important also fixing plants in Western China. In recent years, with an increase in economic benefits and resource requirements, the wild licorice resources of china have been uncontrolled excavation, the total amount and the quality of resources is declining.It is the main way to solve the above problems by cultivating licorice. However,Due to their growth characteristics of licorice, the roots grow to one meter deep underground, and harvesting costs are too high, too much waste,which are major factors to restrict the cultivate of licorice. In this study, we will screen suitable root specific expression promoters of licorice by using molecular biology, and express auxin and cytokinin gene with this promoter-driven in licorice root, then we will analyze the morphology of licorice root, and its impact on growth status. Finially,we find out enlargement of licorice root.Licorice root enlargement is bound to affect the depth of its roots implanted in the soil , but the roots of enlargement will increase the biomass yield largely licorice and shorten the growing cycle. We have established a pre- Ural licorice and Glycyrrhiza inflate regeneration system for the project to carry out this study has laid a good foundation . The results of this research project is expected to solve difficult problems in production on radically improved planting licorice economic benefits, thus promoting the industrialization process of Xinjiang licorice plant industry.

甘草是一种重要的大宗药材，也是我国西部地区重要的固沙植物。近年来在经济利益和资源需求增加的驱动下，我国野生甘草资源遭到无节制的采挖，资源总量和质量逐年下降。人工种植甘草成为解决以上问题的主要途径。由于甘草自身生长特点，根部植于地下一米多深，采收成本过高，浪费过大，是制约甘草人工种植的主要因素。本项目应用现代分子生物学手段，筛选出适合甘草根部全根表达的根部特异性启动子，利用此启动子驱动植物生长素和细胞分裂素基因在甘草根部大量表达，分析其对甘草根部形态及生长状态影响，筛选出根部膨大的甘草。甘草根部膨大必然会影响其根部植入土壤的深度，而且根部膨大在很大程度上会提高甘草的生物产量和缩短种植周期。前期我们已经建立了乌拉尔甘草和胀果甘草再生体系，这为本项目的开展奠定了很好的研究基础。本项目的研究成果有望从根本上解决生产上的难点问题，提高种植甘草经济效益，从而推动新疆甘草种植产业的产业化进程。