生物活性蛋白微球复合凝胶内皮化修复重建颅脑血管的研究

There are some limitations in the course of intervantional embolization of haemorrhagic cerebrovascular diseases by using microcoils(MC). In this study,forcus on the main reason of the reccurrence of postembolized aneurysms(endothelial injury and defects), we want to make manufacture bioactive protein alginate-microsphere after crosslinking CD34 and VEGF with alginate..By using this kind of microsphere, bioactive protein microspheres-based composite gel can be made with other natural biodegradable polymer. Alginate microsphere can protect bioactive protein and incrrease its stablity. Taking MCs as carriers, we transmitted bioactive protein （CD34 antibody or VEGF）microspheres-based composite gel to the target lesion of blood vessels. EPC can reaching the damaged area through circulation and captured by CD34.These effect induce the rapid establishment of a functional endothelial layer on the ECM in the healing response, accelerate endothelialization of damaged vessels. .The MC coated with this kind of gel composite can mechanically block the lesion of blood vessel accompanied with repair and reconstruction vessels, with better ability of capturing EPCs in the circulation, which can grow up to endothelial cell on the surface of the ECM, and grow persistently under the impaction of VEGF and blood flow, gradually rehabilitate the inner wall of the blood vessel. The ingredient of vascular wall with better biological characteristics anatomically heal up the lesion and the function of blood vessels renew.

采用微弹簧圈（MC）经血管内栓塞治疗出血性脑血管病存在某些缺陷。本研究针对于动脉瘤栓塞后复发的主要因素（内皮损伤及缺损），旨在将能够与内皮祖细胞（EPC）结合的CD34抗体及VEGF分别与藻酸盐混合交联后，制备生物活性蛋白藻酸盐微球。利用此微球与其他天然高分子可降解材料合成一种可以作为良好细胞外基质的生物活性蛋白微球复合凝胶。微球化可以将生物活性蛋白与材料合一，对于蛋白能起到一定保护作用，可增加生物活性蛋白的稳定性，使其持续发挥作用。经血管内介入技术将这种生物活性蛋白微球复合凝胶植入于血管病变部位，兼有修复重建及机械闭塞血管缺陷的优势，结合预衬抗体能够在体内直接、持续的发挥作用捕获血液循环中表达特异性抗体的内皮祖细胞（EPC），在VEGF的促进下EPC能够在复合凝胶材料作为细胞外基质的表面持续生长、分化为内皮细胞，使血管病变处被内皮细胞封闭，逐渐内皮化恢复脑血管腔内膜的解剖完整性。

采用微弹簧圈（MC）经血管内栓塞治疗出血性脑血管病存在某些缺陷。本研究针对于动脉瘤栓塞后复发的主要因素（内皮损伤及缺损），旨在将能够与内皮祖细胞（EPC）结合的CD34 抗体及VEGF分别与藻酸盐混合交联后，制备生物活性蛋白藻酸盐微球。利用此微球与其他天然高分子可降解材料合成一种可以作为良好细胞外基质的生物活性蛋白微球复合凝胶。微球化可以将生物活性蛋白与材料合一，对于蛋白能起到一定保护作用，可增加生物活性蛋白的稳定性，使其持续发挥作用。经血管内介入技术将这种生物活性蛋白微球复合凝胶植入于血管病变部位，兼有修复重建及机械闭塞血管缺陷的优势，结合预衬抗体能够在体内直接、持续的发挥作用捕获血液循环中表达特异性抗体的内皮祖细胞（EPC），在VEGF 的促进下EPC 能够在复合凝胶材料作为细胞外基质的表面持续生长、分化为内皮细胞，使血管病变处被内皮细胞封闭，逐渐内皮化恢复脑血管腔内膜的解剖完整性。