蛋白乳液-阴离子多糖凝胶微球界面蛋白结构变化与体系稳定性机制研究

The structure change of oil/water interfacial protein is an important factor on the stability of protein-emulsion delivery system. It has become a trend to apply new techniques in proteomics instead of traditional spectroscopy to analyze the fine structure of oil/water interfacial proteins. Protein emulsion - anionic polysaccharide gel microbeads is a novel delivery system with amphiphilic substance entrapment, which has been widely applied in the bioactive compounds delivery in food field. While there still exiting prominent problems in the fabrication process, such as unclear structure changes and interface instability. In this project, the mixture of stable β-lactoglobulin emulsion and anionic polysaccharide sodium alginate was injected into calcium ion solution-based emulsion-microencapsulation. Analyze the macro and fine structure changes and interfacial property of oil/water interfacial protein under different ratio of polysaccharide and protein, concentration and pH by hydrogen/deuterium exchange mass spectrometry technique (HDX-MS) evaluate the effect of fabrication parameters on stability and digestion of delivery systems, elucidate the intrinsic rules of effect of fabrication parameters, protein structure stability, and clarify the structure changes of oil/water interfacial protein and the mechanism of delivery system stability. This research provides a theoretical basis for the fabrication of interfacial structural stability and steady-state emulsion - gel microbeads delivery system.

油-水界面蛋白结构变化是影响蛋白基-乳液递送体系稳定性的重要因素，应用蛋白组学领域新技术替代传统光谱学技术解析油-水界面蛋白精细结构具有重要意义。蛋白乳液-阴离子多糖凝胶微球是一种新型乳液基双亲性物质递送体系，已广泛应用于食品活性物质递送领域，但其在制备过程中存在界面蛋白结构变化不明、界面失稳的突出问题。本项目将β-乳球蛋白乳液与阴离子多糖海藻酸钠混合，微封包注入钙离子溶液制备乳液-凝胶微球。拟采用氢-氘交换质谱技术（HDX-MS）表征不同多糖-蛋白比例、浓度、pH等制备参数下油-水界面蛋白宏观及精细结构变化，并评价制备参数对乳液-凝胶微球稳定性的影响。揭示制备参数、界面蛋白结构变化及其稳定性之间的内在规律，明确递送体系界面蛋白结构变化与体系稳态化机制。上述研究为蛋白基乳液递送体系界面结构解析提供新思路，也可为构建稳态化乳液-凝胶微球递送体系提供理论依据。

蛋白乳液-阴离子多糖凝胶微球是一种新型乳液基双亲性物质递送体系，已广泛应用于食品活性物质递送领域，但其在制备过程中存在界面蛋白结构变化不明、界面失稳的突出问题。本项目团队针对此问题，通过三年的研究，已基本完成项目计划内容。首先采用不同的乳化剂以及多糖-乳液分别制备不同的递送体系，评价递送体系在不同的环境应力条件下稳定的变化，明确主要的影响因素。再通过研究递送体系制备条件对稳定性的影响，明确影响界面失稳的主要因素和调节机制。相关结果已发表SCI JCR1区论文3篇，作为项目负责人获得中国博士后面上项目1项，肇庆市西江创业领军团队项目1项，广东省科技项目2项，广州市基础与应用基础项目1项。培养研究生4人，参加国际油脂大会2次。