基于化学发光共振能量转移的半导体纳米晶光子晶体多元分析芯片

Semiconductor nanocrystal (quatum dot) has broad applications in biochip because of its rich band structures and excellent optical properties. Chemiluminescence resonance energy transfer (CRET) takes lots of attentions in analysis field because it has non background influence and low fluorescent bleach. This research focus on the enhancement of fluorescent intensity and improvement of detection sensitivity of chemiluminescence resonance energy transfer process by modifying the DNA based on CRET process on photonic crystal (PC) which has varied stopband. Besides, high-sensitive, multivariate and high-flux analysis of cancer will be achieved by quantum dots with different colors and specific cancer markers. Moreover, the role of PC in enhancement CRET process and the life of reactive intermediates will be well discussed. This work will expand the applications of PC biochip, benefit to systematically study the relationship between PC and CRET, and provide theoretical and technical foundation with independent intellectual property rights for the development of new sensors and detection devices based on CRET process.

半导体纳米晶（量子点）具有丰富的能带结构和良好的光学性质，在生物芯片等领域有广泛的应用。化学发光共振能量转移体系因其无背景干扰、低荧光漂白等优点在分析领域受到广泛的关注。本研究将利用光子晶体的禁带效应，在光子晶体表面上，利用化学方法修饰基于化学发光共振能量转移原理的DNA分子，通过调节光子晶体的带隙，增强荧光信号，提升检测的灵敏度。同时，利用不同颜色的量子点以及具有不同特异性识别功能的DNA对多种的癌症标志物进行检测，实现高灵敏、多组分、高通量的癌症筛选。此外，通过调节光子晶体带隙来进一步研究光子晶体在增强化学发光共振能量转移过程中的作用，特别是对反应活性中间体寿命的影响。该研究将大大拓展DNA光子晶体芯片的适用范围，有利于系统研究光子晶体与化学发光共振能量转移之间的关系，为基于化学发光共振能量转移原理的新型传感和检测器件的发展提供具有自主知识产权的理论和技术基础。

光子晶体传感器的研究重点从基本原理和材料的开发转向器件化、芯片化、集成化。本项目将喷墨打印的方法和纳米粒子自组装相结合，实现光子晶体的简单、快速、大面积图案化组装，并研究了功能性光子晶体在图案化可控组装后在光学、传感等领域的潜在应用。系统研究光子晶体荧光传感器和色度传感器的工作原理, 并结合近年来光子晶体传感器研究的新进展提出了光子晶体传感器发展的新思路。项目期间在Sci. Adv., Nat. Comm, Angew. Chem. Int. Ed., Adv. Energy Mater., Small等重要学术期刊发表SCI 收录论文19篇，申请专利5项。