基于对β-淀粉样蛋白代谢酶调控作用的地黄饮子防治AD研究

β amyloid protein metabolic abnormalities is A central part of the pathological mechanism of Alzheimer's diseases,reduce Aβ in the brain produce and promoteAβ and suppress Aβ gathered in order to reduce its neurotoxicity is the new direction of Preventing And Therapy AD ..Traditional Chinese medicine theory is that the basic pathogenesis of AD is kidney empty phlegm obstruction, dihuang Yinzi decoction is a classic party of kidney and phlegm, basis of ancient and modern clinical reports have shown the AD treatment have good curative effect. The extensive studies confirm that dihuang Yinzi Decoction can through the blood brain barrier, direct effects on the brain lesion, can obviously inhibit the excessive expression of APPmRNA, shows its inhibition of A good prospect of beta deposition..This project is to control Aβ metabolic enzymes for strategy, adopt the internationally recognized as A kind of transgenic mice and two kinds of transgenic cells, from Aβ generated and degradation pathway system to study the effect of radix dihuang Yinzi decoction suppress Aβ deposition targets. This study further system objectively evaluate the mechanism of action of radix dihuang Yinzi , looking for radixdihuang Yinzi decoction targets for the prevention and treatment of AD, for the prevention and treatment of AD classic new drug development research foundation.A central part of the pathological mechanism.

β淀粉样蛋白(β-amyloid protein,Aβ) 代谢异常是老年性痴呆（Alzheimer’s disease,AD）病理机制的中心环节，减少Aβ在脑内的产生、促进Aβ清除、抑制Aβ聚集以降低其神经毒性是AD治疗的新方向。.中医学理论认为AD的基本病机为肾虚痰阻，地黄饮子是补肾化痰的经典方，古代医案和现代临床报道均显示其对AD治疗有很好疗效。前期大量研究证实，地黄饮子能够通过血脑屏障，直接作用于大脑病变组织，能明显抑制APPmRNA的过度表达，显示了其抑制Aβ沉积的良好前景。.本项目以调控Aβ代谢酶活性为策略，采用国际公认的一种转基因小鼠和两种转基因细胞，应用ELISA、Western Blot等技术方法，从Aβ生成和降解途径系统探讨地黄饮子抑制Aβ沉积的作用靶点。本研究进一步系统客观地评价地黄饮子的作用机制，寻找地黄饮子防治AD的作用靶点，为防治AD的经典方新药开发研究奠定基础。

本研究根据Aβ代谢异常这一病理中心环节，着眼Aβ的代谢过程，从Aβ代谢酶入手，通过实验，以两种转基因细胞为靶点，通过观察地黄饮子对Aβ代谢酶的调节作用，研究地黄饮子对Aβ生成和降解过程的影响。. 结果发现地黄饮子对影响Aβ沉积的代谢酶具有抑制作用，对促进Aβ降解的分泌酶具有促进作用。为进一步深入系统地探讨补肾化痰方药地黄饮子防治AD的疗效及机制提供了重要的理论依据，为治疗AD的经典方新药开发研究奠定基础。有利于理解并充实中医的脑髓学说，揭示AD的中医病因病机，为补肾化痰法治疗神经退行性疾病提供了依据。